Genome-wide analysis of a land plant-specific acyl:coenzyme A synthetase (ACS) gene family in Arabidopsis, poplar, rice and Physcomitrella.
Genome-wide analysis of a land plant-specific acyl:coenzyme A synthetase (ACS) gene family in Arabidopsis, poplar, rice and Physcomitrella.
复制标题
DOI：
10.1111/j.1469-8137.2008.02534.x
复制
发表时间：
2008-09
期刊：
影响因子：
--
通讯作者：
Clarice de Azevedo Souza;Brad Barbazuk;S. Ralph;J. Bohlmann;B. Hamberger;C. Douglas
中科院分区：
文献类型：
--
作者：
Clarice de Azevedo Souza;Brad Barbazuk;S. Ralph;J. Bohlmann;B. Hamberger;C. Douglas
植物酶 4-香豆酸：辅酶 A 连接酶 (4CL) 是所有生物体中存在的腺苷酸形成酶家族的一部分。基因组序列分析表明植物和其他生物体中存在“4CL 样”酶，但它们的进化关系和功能仍然很大程度上未知。通过 BLAST 搜索在拟南芥、杨树、水稻、立碗藻、衣藻和微生物基因组中鉴定出 4CL 和 4CL 样基因。通过比对氨基酸序列的系统发育分析推断进化关系。分析了一组保守的拟南芥和杨树 4CL 样酰基辅酶 A 合成酶 (ACS) 基因的表达模式。保守的 ACS 基因形成陆地植物特有的类别。被子植物 ACS 基因分为五个分支，每个分支包含三个完全测序的基因组中的代表。表达分析揭示了一些进化枝中拟南芥和杨树基因的保守发育和胁迫诱导表达模式。植物 ACS 酶的进化发生在陆地植物的早期。被子植物 ACS 进化枝的差异基因扩增已在某些谱系中发生。进化和基因表达数据，结合体外和有限的体内蛋白质功能数据，表明被子植物 ACS 酶在十八烷和脂肪酸代谢中发挥保守作用，并在器官发育中发挥作用，例如在花药中。
The plant enzyme 4-coumarate:coenzyme A ligase (4CL) is part of a family of adenylate-forming enzymes present in all organisms. Analysis of genome sequences shows the presence of '4CL-like' enzymes in plants and other organisms, but their evolutionary relationships and functions remain largely unknown. 4CL and 4CL-like genes were identified by BLAST searches in Arabidopsis, Populus, rice, Physcomitrella, Chlamydomonas and microbial genomes. Evolutionary relationships were inferred by phylogenetic analysis of aligned amino acid sequences. Expression patterns of a conserved set of Arabidopsis and poplar 4CL-like acyl-CoA synthetase (ACS) genes were assayed. The conserved ACS genes form a land plant-specific class. Angiosperm ACS genes grouped into five clades, each of which contained representatives in three fully sequenced genomes. Expression analysis revealed conserved developmental and stress-induced expression patterns of Arabidopsis and poplar genes in some clades. Evolution of plant ACS enzymes occurred early in land plants. Differential gene expansion of angiosperm ACS clades has occurred in some lineages. Evolutionary and gene expression data, combined with in vitro and limited in vivo protein function data, suggest that angiosperm ACS enzymes play conserved roles in octadecanoid and fatty acid metabolism, and play roles in organ development, for example in anthers.