A single re-implanted ventral root exerts neurotropic effects over multiple spinal cord segments in the adult rat.
A single re-implanted ventral root exerts neurotropic effects over multiple spinal cord segments in the adult rat.
复制标题
单个重新植入的腹侧根对成年大鼠的多个脊髓节段发挥神经营养作用。
DOI：
10.1007/s00221-005-0137-4
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发表时间：
2006
影响因子：
2
通讯作者：
Havton,LeifA
中科院分区：
文献类型：
--
作者：
Hoang,ThaoX;Havton,LeifA
脊髓损伤，特别是脊髓圆锥和马尾的创伤性损伤，通常是复杂的，涉及多个节段水平。将撕脱的前根植入脊髓作为修复策略已被证明具有神经保护作用，并促进脊髓神经元轴突再生到植入的根中。然而，目前还不清楚植入的前根在脊髓中的距离可以发挥其亲神经性作用。在这里，我们调查是否撕脱的L 6腹根急性植入大鼠脊髓后，四个水平（L5-S2）单侧腹根撕脱损伤可能会产生神经营养作用的自主神经元和运动神经元在多个脊髓节段在术后6周。使用逆行标记技术和体视学定量方法，我们证明，自主神经和运动神经元从所有四个病变的脊髓段，跨越超过8毫米的吻尾距离，再神经支配的一个植入的根。的喙尾分布建议的神经营养性的梯度，其中轴突切断神经元最接近植入部位的根神经再支配的可能性最高。这些结果表明，植入一个单一的前根可能提供神经营养作用的损伤神经元在病变部位以及在相邻的脊髓节段。我们的研究结果可能是翻译的研究兴趣的发展后，多个层面的脊髓圆锥和马尾神经损伤，其中前根比脊髓节段可用于植入的手术修复策略。
Spinal cord injuries, particularly traumatic injuries to the conus medullaris and cauda equina, are typically complex and involve multiple segmental levels. Implantation of avulsed ventral roots into the spinal cord as a repair strategy has been shown to be neuroprotective and promote axonal regeneration by spinal cord neurons into an implanted root. However, it is not well known over what distance in the spinal cord an implanted ventral root can exert its neurotropic effect. Here, we investigated whether an avulsed L6 ventral root acutely implanted into the rat spinal cord after a four level (L5–S2) unilateral ventral root avulsion injury may exert neurotropic effects on autonomic and motor neurons over multiple spinal cord segments at 6 weeks postoperatively. Using retrograde labeling techniques and stereological quantification methods, we demonstrate that autonomic and motor neurons from all four lesioned spinal cord segments, spanning more than an 8 mm rostro-caudal distance, reinnervated the one implanted root. The rostro-caudal distribution suggested a gradient of neurotropism, where the axotomized neurons closest to the implanted site had the highest probability of root reinnervation. These results suggest that implantation of a single ventral root may provide neurotropic effects to injured neurons at the site of lesion as well as in the adjacent spinal cord segments. Our findings may be of translational research interest for the development of surgical repair strategies after multi-level conus medullaris and cauda equina injuries, in which fewer ventral roots than spinal cord segments may be available for implantation.