Unusual abundance of vertebrate 3-phosphate dehydrogenase pseudogenes
Unusual abundance of vertebrate 3-phosphate dehydrogenase pseudogenes
复制标题
脊椎动物 3-磷酸脱氢酶假基因异常丰度
作者：
M. Piechaczyk;J. Blanchard;S. E. Sabouty;C. Dani;L. Marty;P. Jeanteur
已知只有一个编码甘油醛3-磷酸脱氢酶（GAPDH，EC 1.2.1.12）（控制糖酵解的关键酶）的基因在人、小鼠、大鼠和鸡中起作用1 -6。该基因已定位于人的12号染色体2、3和小鼠的6号染色体4。在鸡7 -9和大鼠10中只发现了单一的mRNA种类。然而，用克隆的GAPDH cDNA探针对不同物种的基因组DNA印迹进行分析，发现重复水平存在很大差异，范围从1个拷贝到超过200个拷贝。在此基础上，我们根据这些生物体所包含的GAPDH相关序列的数量将其分为三类;一类具有独特的代表性（鸡），另一类相对较低的重复（人、野兔、豚鼠和仓鼠中有10-30个拷贝）和第三类高重复（小鼠和大鼠中>200个拷贝）。第三类代表了第一次报道的与酶编码基因相关的如此极端数量的假基因的出现，并表明在1500万至2500万年前发生了戏剧性的扩增事件。
Only one gene coding for glyceraldehyde 3-phosphate dehydrogenase (GAPDH, EC 1.2.1.12), a key enzyme in the control of glycolysis, is known to be functional in man, mouse, rat and chicken1–6. The gene has been localized to chromosome 12 in human2,3 and chromosome 6 in mouse4. Only a single mRNA species has been found in chicken7–9 and rat10. However, analysis of genomic DNA blots of various species with a cloned GAPDH cDNA probe has revealed large differences in the level of reiteration, ranging from one to over 200 copies. On this basis, we have grouped these organisms into three classes according to the number of GAPDH-related sequences they contain; one class with a unique representation (chicken), another class of relatively low reiteration (10–30 copies in man, hare, guinea-pig and hamster) and a third class of high reiteration (>200 copies in mouse and rat). The third class represents the first reported occurrence of such an extreme number of pseudogenes related to an enzyme-coding gene and suggests that a dramatic amplification event took place between 15 and 25 million years ago.
精氨基琥珀酸合成酶 mRNA cDNA 的克隆以及人类细胞系中酶过量生产的研究。
DOI：
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发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Su,TS;Bock,HG;O'Brien,WE;Beaudet,AL
通讯作者：
Beaudet,AL