A novel DPP6 isoform (DPP6-E) can account for differences between neuronal and reconstituted A-type K(+) channels.
A novel DPP6 isoform (DPP6-E) can account for differences between neuronal and reconstituted A-type K(+) channels.
复制标题
DOI：
10.1016/j.neulet.2008.10.098
复制
发表时间：
2009-01-16
影响因子：
2.5
通讯作者：
Rudy B
中科院分区：
文献类型：
--
作者：
Maffie J;Blenkinsop T;Rudy B
神经元体树突状A-型钾电流的通道主要由Kv4致孔亚基和两种辅助亚基组成的三元复合体，即K+通道相互作用蛋白(KChIPs)和二肽基肽酶样蛋白(DPPL)。Kv4.2、KChIP1和DPP6-S在异源表达系统中表达的通道在许多特性上类似于海马CA1区锥体细胞和小脑颗粒细胞的A型电流，这些神经元具有显著的A型K+电流。然而，自然电流具有更快的动力学。此外，上丘中间层神经元的A型电流具有更快的失活率。我们鉴定了一种新的DPP6剪接异构体DPP6-E，它在异种细胞中以非常快的动力学方式产生三元Kv4通道。DPP6-E选择性表达于小脑颗粒神经元、海马锥体细胞和上丘中间层神经元。DPP6-E的作用解释了一些神经元中重组Kv4通道和天然Kv4通道之间过去的差异，并有助于神经元中A型K+电流的多样性。
The channels mediating most of the somatodendritic A-type K+ current in neurons are thought to be ternary complexes of Kv4 pore-forming subunits and two types of auxiliary subunits, the K+ channel interacting proteins (KChIPs) and dipeptidyl-peptidase-like (DPPL) proteins. The channels expressed in heterologous expression systems by mixtures of Kv4.2, KChIP1 and DPP6-S resemble in many properties the A-type current in hippocampal CA1 pyramidal neurons and cerebellar granule cells, neurons with prominent A-type K+ currents. However, the native currents have faster kinetics. Moreover, the A-type currents in neurons in intermediary layers of the superior colliculus have even faster inactivating rates. We have characterized a new DPP6 spliced isoform, DPP6-E, that produces in heterologous cells ternary Kv4 channels with very fast kinetics. DPP6-E is selectively expressed in a few neuronal populations in brain including cerebellar granule neurons, hippocampal pyramidal cells and neurons in intermediary layers of the superior colliculus. The effects of DPP6-E explain past discrepancies between reconstituted and native Kv4 channels in some neurons, and contributes to the diversity of A-type K+ currents in neurons.
登录
查看更多内容
影响因子：
3.5
作者：
Jerng, Henry H.;Lauver, Aaron D.;Pfaffinger, Paul J.
通讯作者：
Pfaffinger, Paul J.
影响因子：
5.5
作者：
Korngreen, A;Sakmann, B
通讯作者：
Sakmann, B
影响因子：
5.5
作者：
Bekkers, JM
通讯作者：
Bekkers, JM
影响因子：
2.9
作者：
Nadal, Marcela S.;Amarillo, Yimy;Rudy, Bernardo
通讯作者：
Rudy, Bernardo
影响因子：
5.3
作者：
Malin, SA;Nerbonne, JM
通讯作者：
Nerbonne, JM