Rapid separation of microbial lipids using solid phase extraction columns
Rapid separation of microbial lipids using solid phase extraction columns
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DOI：
10.1016/s0167-7012(98)00060-8
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发表时间：
1998-09-01
影响因子：
2.2
通讯作者：
Chapman, PJ
中科院分区：
文献类型：
--
作者：
Pinkart, HC;Devereux, R;Chapman, PJ
建立了一种快速分离微生物中常见脂类的方法。该方法是基于使用氨丙基固相萃取柱分离聚羟基烷酸酯（PHA），磷脂，甾醇，甘油三酯，二甘油三酯，单甘油三酯和甾酯。除PHA和甾醇外，所有脂类的回收率从91%到大于99%不等。PHA回收率为标准的69%，甾醇回收率为标准的82-84%。将该方法应用于蓝藻螺旋藻的脂质分析，对饱和脂肪酸、单不饱和脂肪酸和多不饱和脂肪酸等磷脂和二甘油酯具有良好的回收和分离效果。此外，白檀脂质中还分别含有甾酯和甘油三酯馏分中回收的烃类和叶绿醇。该方法具有产率高、特异性强、分离速度快、与其他脂类污染少的特点，可用于环境样品中微生物群落的表征。(C) 1998 Elsevier Science B.V.版权所有
A method was developed to rapidly separate lipid classes commonly found in microorganisms. The method is based on the use of aminopropyl solid phase extraction columns to separate polyhydroxyalkanoates (PHA), phospholipids, sterols, triglycerides, diglycerides, monoglycerides, and steryl esters. Recoveries of all lipid classes, with the exception of PHA and sterols, ranged from 91% to greater than 99%. PHA were recovered at 69% of the standard, and sterols from 82-84% of the standard. When applied to the analysis of lipids extracted from the cyanobacterium Spirulina platensis, the method afforded excellent recovery and separation of phospholipids and diglycerides including saturated, monounsaturated and polyunsaturated fatty acids. The S. platensis lipids also contained hydrocarbons and phytol recovered in the steryl ester and diglyceride fractions, respectively. This method provided a high yield, specific and rapid separation of microbial lipids with little contamination from other lipid groups, and will be useful for the characterization of microbial communities in environmental samples. (C) 1998 Elsevier Science B.V. All rights reserved.