DETECTION OF HEPATITIS-B VIRUS-DNA IN MONONUCLEAR BLOOD-CELLS
DETECTION OF HEPATITIS-B VIRUS-DNA IN MONONUCLEAR BLOOD-CELLS
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DOI：
10.1136/bmj.288.6430.1563
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发表时间：
1984-01-01
影响因子：
105.7
通讯作者：
BRECHOT, C
中科院分区：
文献类型：
--
作者：
PONTISSO, P;POON, MC;BRECHOT, C
应用Southern转移杂交技术检测单个核细胞中的B型肝炎病毒DNA。16例B型肝炎病毒感染者中10例单个核细胞中检测到病毒DNA序列，21例正常对照者中无一例检测到病毒DNA序列。所有病例的相应血清样本中均不存在B型肝炎病毒DNA，排除了血液污染。3例e抗原（HBeAg）阳性和1例抗-HBe阳性的患者检出游离单体型HBV DNA，4例e抗体（抗-B）阳性的患者检出整合型HBV DNA。在另外2名患者中，样本量小，无法区分游离和整合的病毒DNA。单核细胞中病毒的状态似乎与HBeAg或抗-HBe状态相关，正如在肝脏中所观察到的那样。显然，B型肝炎病毒可感染单核血细胞，从而将该试剂的组织特异性扩展到肝脏以外，如已报道的胰腺、肾脏和皮肤组织。他们还认为，B型肝炎病毒感染单核细胞可能与病毒携带者的免疫异常有关。
The Southern transfer hybridization technique was used to test mononuclear blood cells for hepatitis B virus DNA. Viral DNA sequences were detected in mononuclear cells of 10 of 16 patients with hepatitis B virus infection and in none of 21 normal controls. Blood contamination was excluded by the absence of hepatitis B virus DNA in the corresponding serum samples in all cases. Free monomeric hepatitis B virus DNA was found in 3 patients positive for hepatitis B e antigen (HBeAg) and 1 positive for anti-HBe, and integrated hepatitis B virus DNA was present in 4 patients positive for anti-hepatitis B e antibody (anti-HBe). In 2 other patients the small size of the samples did not allow a distinction between free and integrated viral DNA. The state of the virus in the mononuclear cells seemed to correlate with the HBeAg or anti-HBe state, as has been noted in the liver. Evidently, hepatitis B virus may infect mononuclear blood cells, thereby expanding the tissue specificity of this agent beyond the liver, as has been reported for pancreatic, kidney and skin tissue. They also suggest that hepatitis B virus infection of mononuclear cells might be related to immunological abnormalities observed in carriers of the virus.