Nanophotonic and hydrogel-based diagnostic system for the monitoring of chronic wounds
Nanophotonic and hydrogel-based diagnostic system for the monitoring of chronic wounds
复制标题
基于纳米光子和水凝胶的诊断系统，用于监测慢性伤口
DOI：
10.1016/j.bios.2023.115743
复制
发表时间：
2023
影响因子：
12.6
通讯作者：
Bakshi S
中科院分区：
文献类型：
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作者：
Bakshi S
慢性伤口是一个主要的医疗负担，然而大多数伤口只进行了表面评估，治疗很少基于伤口生物标志物的分析。缺乏分析是基于这样一个事实，即伤口生物标记物的采样通常是有创的，导致伤口床被破坏，而生物标记物的检测和量化是在远离护理地点的远程实验室进行的。在这里，我们介绍了一种新型的治疗系统的诊断元件，该系统可以在不破坏伤口的情况下非侵入性地采样生物标记物，并且可以在护理点在短时间内执行生物标记物的量化。该系统基于一种可热切换的水凝胶支架，它通过伤口组织的再生促进伤口愈合，并允许非破坏性地提取伤口生物标记物。我们证明了两个伤口健康的主要生物标志物，即IL-6和肿瘤坏死因子-α，在吸收到水凝胶敷料中的人体基质中被检测到。直接定量水凝胶中的生物标志物是使用啁啾导模共振生物传感器实现的，我们展示了在临床相关的pg/m L到μg/m L浓度范围内生物标记物的检测。我们还用临床伤口渗出液样本对水凝胶敷料中浓度为1 ng/mL的IL-6和肿瘤坏死因子-α进行了检测。我们展示的高灵敏度和宽动态范围对于我们的系统的临床相关性都是必不可少的。我们的测试对开发一种用于指导治疗和管理慢性伤口的伤口治疗方法做出了重大贡献。
Chronic wounds present a major healthcare burden, yet most wounds are only assessed superficially, and treatment is rarely based on the analysis of wound biomarkers. This lack of analysis is based on the fact that sampling of wound biomarkers is typically invasive, leading to a disruption of the wound bed while biomarker detection and quantification is performed in a remote laboratory, away from the point of care. Here, we introduce the diagnostic element of a novel theranostic system that can non-invasively sample biomarkers without disrupting the wound and that can perform biomarker quantification at the point of care, on a short timescale. The system is based on a thermally switchable hydrogel scaffold that enhances wound healing through regeneration of the wound tissue and allows the extraction of wound biomarkers non-destructively. We demonstrate the detection of two major biomarkers of wound health, i.e., IL-6 and TNF-α, in human matrix absorbed into the hydrogel dressing. Quantification of the biomarkers directly in the hydrogel is achieved using a chirped guided mode resonant biosensor and we demonstrate biomarker detection within the clinically relevant range of pg/mL to μg/mL concentrations. We also demonstrate the detection of IL-6 and TNF-α at concentration 1 ng/mL in hydrogel dressing absorbed with clinical wound exudate samples. The high sensitivity and the wide dynamic range we demonstrate are both essential for the clinical relevance of our system. Our test makes a major contribution towards the development of a wound theranostic for guided treatment and management of chronic wounds.