Prevention of small intestinal ischemia-reperfusion injury in rat by anti-cytokine-induced neutrophil chemoattractant monoclonal antibody.
Prevention of small intestinal ischemia-reperfusion injury in rat by anti-cytokine-induced neutrophil chemoattractant monoclonal antibody.
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抗细胞因子诱导的中性粒细胞趋化单克隆抗体预防大鼠小肠缺血再灌注损伤。
DOI：
10.1006/jsre.1998.5367
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
K. Hirata
中科院分区：
文献类型：
--
作者：
A. Yagihashi;T. Tsuruma;K. Tarumi;T. Kameshima;T. Yajima;Y. Yanai;N. Watanabe;K. Hirata
胡萝卜素诱导的中性粒细胞趋化因子（CINC）是属于CXC趋化因子亚群的人类生长相关基因产物的大鼠对应物，其功能主要基于嗜中性粒细胞特异性趋化活性。此外，我们以前报道过，血浆CINC在小肠缺血再灌注损伤期间升高，并且再灌注后粘膜损伤程度与CINC峰值水平之间存在相关性，提示CINC可能在中性粒细胞浸润到大鼠小肠缺血再灌注损伤部位中起主要作用。因此，我们研究了施用抗CINC单克隆抗体（mAb）是否减少小肠缺血-再灌注损伤。小肠缺血3小时，通过闭塞肠系膜前动脉与无创伤性血管丛。在输注抗CINC mAb或同种型匹配的mAb后，对肠进行再灌注。抗CINC单克隆抗体预处理可减轻小肠缺血再灌注损伤，减少肿瘤坏死因子-α和髓过氧化物酶的产生，并导致生存期延长。结论：CINC在大鼠小肠缺血再灌注损伤的发生中起重要作用。此外，阻断CINC的作用，即中性粒细胞趋化活性，可能有助于防止小肠缺血-再灌注损伤。
The function of cytokine-induced neutrophil chemoattractant (CINC), which is the rat counterpart to human growth-related gene product belonging to the CXC chemokine subgroup, is based principally on neutrophil-specific chemotactic activity. In addition, we previously reported that plasma CINC was elevated during the period of small intestinal ischemia-reperfusion injury, and that there was a correlation between the degree of mucosal damage and the peak level of CINC after reperfusion, suggesting that CINC may play a major role in neutrophil infiltration into the rat small intestinal ischemia-reperfusion injury site. Thus, we investigated whether administration of anti-CINC monoclonal antibodies (mAbs) reduces small intestinal ischemia-reperfusion injury. Small intestine was subjected to ischemia for 3 h by occlusion of the anterior mesenteric artery with an atraumatic vascular clump. After infusion of anti-CINC mAbs or isotype-matched mAbs, the intestine was subjected to reperfusion. The pretreatment with anti-CINC mAbs attenuated ischemia-reperfusion injury in the small intestine, in association with the reduction of tumor necrosis factor-alpha and myeloperoxidase production, and resulted in the prolongation of survival. It is concluded that CINC plays an important role in the onset of rat small intestinal ischemia-reperfusion injury. In addition, blocking the action of CINC, namely, the neutrophil chemotactic activity, may be useful in preventing ischemia-reperfusion injury in the small intestine.
DOI：
10.1073/pnas.82.24.8667
发表时间：
1985-12-01
影响因子：
11.1
作者：
GAMBLE, JR;HARLAN, JM;VADAS, MA
通讯作者：
VADAS, MA