IMMUNE EVENTS IN SKIN .1. SPONTANEOUS CLUSTER FORMATION OF DENDRITIC (VEILED) CELLS AND LYMPHOCYTES FROM SKIN LYMPH
IMMUNE EVENTS IN SKIN .1. SPONTANEOUS CLUSTER FORMATION OF DENDRITIC (VEILED) CELLS AND LYMPHOCYTES FROM SKIN LYMPH
复制标题
DOI：
10.1111/j.1365-3083.1992.tb02981.x
复制
发表时间：
1992-06-01
影响因子：
3.7
通讯作者：
OLSZEWSKI, WL
中科院分区：
文献类型：
--
作者：
GALKOWSKA, H;OLSZEWSKI, WL
为了研究传入淋巴细胞自发附着于树突状细胞的机制，使用了来自犬皮肤淋巴的细胞。在从插管淋巴管流出的淋巴液中发现了 3.3 +/- 2.8% 的簇状遮盖细胞。在收集的淋巴样本中离体形成的簇的数量作为时间的函数而增加并且与温度相关。将细胞与蛋白水解酶或单糖一起孵育不会改变细胞相互作用。被遮蔽的细胞结合淋巴细胞的能力与二价阳离子无关，但会被赛洛卡因和视黄酸降低。在类固醇中，只有甲基强的松龙显示出对簇形成的抑制作用。吲哚美辛和乙酰水杨酸对细胞结合没有阻断活性。此外，用环孢素A和硫唑嘌呤治疗后未见效果。观察到用神经氨酸酶去唾液酸化后簇形成增强。培养去唾液酸化的细胞以研究它们的刺激和辅助细胞功能。没有观察到自体混合白细胞反应的增强，但观察到对次优剂量的植物血凝素的反应性明显较高。 N-酶介导的非特异性细胞附着可以通过细胞洗涤或用 EDTA 或利多卡因处理来消除。这项研究表明，皮肤淋巴覆盖细胞和淋巴细胞的簇形成是一个自发过程，不能通过通常有效调节体外诱导的抗原刺激细胞簇的方法来控制。
To investigate the mechanism of spontaneous attachment of afferent lymph lymphocytes to dendritic cells, cells from canine skin lymph were used. There were 3.3 +/- 2.8% of veiled cells in clusters found in lymph flowing from the cannulated lymph vessel. The number of clusters forming ex vivo in the collected lymph samples increased as a function of time and was temperature dependent. Incubation of cells with proteolytic enzymes or monosaccharides did not alter cell interactions. The ability of veiled cells to bind lymphocytes was independent of divalent cations but reduced by xylocaine and retinoic acid. Among steroids only methylprednisolone showed an inhibitory effect on cluster formation. Indomethacin and acetylsalicylic acid had no blocking activity on cell binding. Also, no effect was seen after treatment with cyclosporine A and azathioprine. An enhanced cluster formation after desialylation with neuraminidase was observed. The desialylated cells were cultured in order to study their stimulatory and accessory cell functions. No enhancement of autologous mixed leucocyte reaction was seen, but a significantly higher responsiveness to a suboptimal dose of phytohaemagglutinin was observed. The N-ase-mediated non-specific cell attachment could be abrogated by cell washing or treatment with EDTA or xylocaine.This study indicates that cluster formation by skin lymph veiled cells and lymphocytes is a spontaneous process which cannot be controlled by means usually effective in regulating the in vitro induced clustering of antigen-stimulated cells.