Dual Recognition Unit Strategy Improves the Specificity of the Adenosine Triphosphate (ATP) Aptamer Biosensor for Cerebral ATP Assay
Dual Recognition Unit Strategy Improves the Specificity of the Adenosine Triphosphate (ATP) Aptamer Biosensor for Cerebral ATP Assay
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双识别单元策略提高了三磷酸腺苷 (ATP) 适体生物传感器用于脑 ATP 检测的特异性
DOI：
10.1021/ac504249k
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发表时间：
2015
影响因子：
7.4
通讯作者：
Mao Lanqun
中科院分区：
文献类型：
--
作者：
Yu Ping;He Xiulan;Zhang Li;Mao Lanqun
三磷酸腺苷（Adenosine triphosphate，ATP）适配体作为生物传感器的识别单元已得到广泛应用，但其对5′-二磷酸腺苷（adenosine-5′-diphosphate，ADP）和5′-单磷酸腺苷（adenosine-5′-monophosphate，AMP）的特异性较差，限制了其在真实的系统中的应用，尤其是在复杂的脑系统中。在这项研究中，我们首次展示了一个双识别单元策略（DRUS），构建一个高度选择性和敏感的ATP生物传感器相结合的识别能力的适配体对A核碱基和聚咪唑对磷酸盐。该生物传感器首先通过表面引发原子转移自由基聚合（SI-ATRP）将聚咪唑限制在金表面，然后通过静电自组装将适体限制在电极表面，形成双识别单元功能化电极。基于DRUS构建的生物传感器不仅对ATP具有灵敏度高、检测限低至亚阿摩尔水平的特点，而且对ATP具有选择性高、不受ADP和AMP干扰的特点。所构建的生物传感器结合活体微透析技术，用于选择性、灵敏地传感脑系统细胞外ATP，可作为一种有前途的探测脑ATP浓度的神经技术。
Adenosine triphosphate (ATP) aptamer has been widely used as a recognition unit for biosensor development; however, its relatively poor specificity toward ATP against adenosine-5′-diphosphate (ADP) and adenosine-5′-monophosphate (AMP) essentially limits the application of the biosensors in real systems, especially in the complex cerebral system. In this study, for the first time, we demonstrate a dual recognition unit strategy (DRUS) to construct a highly selective and sensitive ATP biosensor by combining the recognition ability of aptamer toward A nucleobase and of polyimidazolium toward phosphate. The biosensors are constructed by first confining the polyimidazolium onto a gold surface by surface-initiated atom transfer radical polymerization (SI-ATRP), and then the aptamer onto electrode surface by electrostatic self-assembly to form dual-recognition-unit-functionalized electrodes. The constructed biosensor based on DRUS not only shows an ultrahigh sensitivity toward ATP with a detection limit down to the subattomole level but also an ultrahigh selectivity toward ATP without interference from ADP and AMP. The constructed biosensor is used for selective and sensitive sensing of the extracellular ATP in the cerebral system by combiningin vivomicrodialysis and can be used as a promising neurotechnology to probing cerebral ATP concentration.