The cellular distribution and oxidation state of platinum(II) and platinum(IV) antitumour complexes in cancer cells
The cellular distribution and oxidation state of platinum(II) and platinum(IV) antitumour complexes in cancer cells
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DOI：
10.1007/s00775-003-0471-6
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发表时间：
2003-09-01
影响因子：
3
通讯作者：
Hambley, TW
中科院分区：
文献类型：
--
作者：
Hall, MD;Dillon, CT;Hambley, TW
铂在A2780卵巢癌细胞中的细胞分布与顺铂和铂（IV）配合物的范围内的还原电位已被检查使用元素分析（同步辐射诱导的X-射线发射）。24小时后铂（IV）药物的细胞分布与顺铂相似，与大多数给药的铂（IV）药物减少一致。用顺铂和铂（IV）络合物处理的细胞的微X射线吸收近边缘光谱证实了铂（IV）还原为铂（II）。在处理的细胞中，在细胞中检测到最难还原的络合物顺式、反式、顺式-[PtCl 2（OH）（2）（NH3）（2）]铂（IV）以及铂（II）。观察结果与所用铂（IV）络合物还原的相对容易性一致，并支持铂（IV）络合物活化需要还原。
The cellular distribution of platinum in A2780 ovarian cancer cells treated with cisplatin and platinum(IV) complexes with a range of reduction potentials has been examined using elemental analysis (synchrotron radiation-induced X-ray emission). The cellular distribution of platinum(IV) drugs after 24 h is similar to that of cisplatin, consistent with the majority of administered platinum(IV) drugs being reduced. Micro-X-ray absorption near-edge spectra of cells treated with cisplatin and platinum(IV) complexes confirmed the reduction of platinum(IV) to platinum(II). In cells treated, the most difficult to reduce complex, cis,trans,cis-[PtCl2(OH)(2)(NH3)(2)], platinum(IV) was detected in the cells along with platinum(II). The observations are in accordance with the relative ease of reduction of the platinum(IV) complexes used and support the requirement of reduction for activation of platinum(IV) complexes.