Demyelination reduces brain parenchymal stiffness quantified in vivo by magnetic resonance elastography
Demyelination reduces brain parenchymal stiffness quantified in vivo by magnetic resonance elastography
复制标题
DOI：
10.1073/pnas.1200151109
复制
发表时间：
2012-04-24
影响因子：
11.1
通讯作者：
Sinkus, Ralph
中科院分区：
文献类型：
--
作者：
Schregel, Katharina;Tysiak, Eva Wuerfel Nee;Sinkus, Ralph
通过手动触诊检测病理组织变化是一种简单但必不可少的诊断工具，自医学诞生以来一直被医生应用。最近，虚拟的“触诊”的大脑已经成为可行的使用磁共振弹性成像，量化的生物力学特性的脑实质通过分析外部引起的剪切波的传播。然而，精确的分子和细胞模式的变化，磁共振弹性成像测量的粘弹性尚未调查到最新。我们评估了多发性硬化症小鼠模型的粘弹性变化，通过喂养铜螯合剂cuprizone诱导可逆性脱髓鞘，并将我们的结果与详细的组织学分析相关联，包括髓鞘形成，细胞外基质改变，免疫细胞浸润和轴突损伤。我们表明，首先，复杂的剪切模量的幅度随着进行性脱髓鞘和全球细胞外基质降解，其次，损耗模量下降速度比动态模量在胼胝体的破坏，最后，这些过程是可逆的髓鞘再生后。
The detection of pathological tissue alterations by manual palpation is a simple but essential diagnostic tool, which has been applied by physicians since the beginnings of medicine. Recently, the virtual "palpation" of the brain has become feasible using magnetic resonance elastography, which quantifies biomechanical properties of the brain parenchyma by analyzing the propagation of externally elicited shear waves. However, the precise molecular and cellular patterns underlying changes of viscoelasticity measured by magnetic resonance elastography have not been investigated up to date. We assessed changes of viscoelasticity in a murine model of multiple sclerosis, inducing reversible demyelination by feeding the copper chelator cuprizone, and correlated our results with detailed histological analyses, comprising myelination, extracellular matrix alterations, immune cell infiltration and axonal damage. We show firstly that the magnitude of the complex shear modulus decreases with progressive demyelination and global extracellular matrix degradation, secondly that the loss modulus decreases faster than the dynamic modulus during the destruction of the corpus callosum, and finally that those processes are reversible after remyelination.