Hepatoprotective effect of ginsenoside Rb1 and compound K on tert-butyl hydroperoxide-induced liver injury
Hepatoprotective effect of ginsenoside Rb1 and compound K on tert-butyl hydroperoxide-induced liver injury
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DOI：
10.1111/j.1478-3231.2005.01068.x
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发表时间：
2005-10-01
影响因子：
6.7
通讯作者：
Kim, DH
中科院分区：
文献类型：
--
作者：
Lee, HU;Bae, EA;Kim, DH
背景/目的：人参的主要化学成分是人参皂苷Rb 1、Rb 2和Rc。口服给药的人参皂苷被肠道细菌代谢为20-O-β-D-吡喃葡萄糖基-20（S）-原人参二醇（化合物K）并被吸收到血液中。为了解其保肝作用及其机制，本研究观察了人参皂苷Rb 1及其代谢产物化合物K对化学损伤的HepG 2细胞和小鼠的影响。方法：从人参中分离得到人参皂苷Rb 1和化合物K。用叔丁基过氧化氢（t-BHP）诱导HepG 2细胞和小鼠的肝毒性。测定HepG 2细胞的细胞毒性和小鼠血清丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）作为保肝活性的标志物。结果：化合物K对t-BHP诱导的HepG 2细胞毒性具有保护作用。然而，NRRb 1并不抑制细胞毒性。然而，当口服给药时，化合物Rb 1和化合物K均显著抑制t-BHP诱导的小鼠ALT和AST升高。但腹腔注射抗坏血酸Rb 1对t-BHP引起的小鼠血浆ALT和AST升高无抑制作用。这些化合物没有表现出抗氧化活性。然而，化合物K显示出比化合物Rb 1更强的膜稳定活性。结论：人参皂苷在肠道中产生的化合物K对肝损伤有保护作用。
Background/Aim: The main component of Panax ginseng, which have been reported by many researchers, are ginsenoside Rb1, Rb2 and Rc. Orally administered ginsenosides are metabolized to 20-O-beta-D-glucopyranosyl-20(S)-protopanaxadiol (compound K) by intestinal bacteria and absorbed to blood. To understand its hepatoprotective effect and its mechanism, the effects of ginsenoside Rb1 and its metabolite compound K on chemically injured HepG2 cells and mice were investigated. Methods: Ginsenoside Rb1 and compound K were isolated from ginseng. Hepatotoxicity of HepG2 cells and mice was induced by tert-butyl hydroperoxide (t-BHP). Cytotoxicity for HepG2 cells and serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) for mice as markers of hepatoprotective activity were measured. Results: Compound K protected HepG2 cell cytotoxicity induced by t-BHP. However, ginsenoside Rb1 did not inhibit cytotoxicity. Nevertheless, both ginsenoside Rb1 and compound K significantly inhibited the increment of ALT and AST induced by t-BHP in mice, when it was orally administered. However, intraperitoneally administered ginsenoside Rb1 did not inhibit the increment of plasma ALT and AST induced by t-BHP in mice. These compounds did not exhibit antioxidant activity. However, compound K showed the potent membrane stabilizing activity more than ginsenoside Rb1. Conclusion: Compound K, which was produced from ginsenosides of Panax ginseng in intestine, could protect liver injury.