Regulation of sulfate uptake, expression of the sulfate transporters Sultr1;1 and Sultr1;2, and APS reductase in Chinese cabbage (Brassica pekinensis) as affected by atmospheric H2S nutrition and sulfate deprivation
Regulation of sulfate uptake, expression of the sulfate transporters Sultr1;1 and Sultr1;2, and APS reductase in Chinese cabbage (Brassica pekinensis) as affected by atmospheric H2S nutrition and sulfate deprivation
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DOI：
10.1071/fp07283
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发表时间：
2008-01-01
影响因子：
3
通讯作者：
De Kok, Luit J.
中科院分区：
文献类型：
--
作者：
Koralewska, Aleksandra;Stuiver, C. Elisabeth E.;De Kok, Luit J.
植物硫酸盐转运蛋白和5‘-磷酸腺苷还原酶(APR)的活性和表达受植物硫素状况和生长需求的调节。为阐明大白菜[Brassica pekinensis(Lour.)研究了大气中H_2S与硫酸盐营养的相互作用及其对硫酸盐转运蛋白和APR活性和表达的影响。在硫酸盐供应充足的条件下，H_2S胁迫使大白菜吸收硫酸盐的能力部分下降，当浓度为-0.25亩L·L~(-1)时，大白菜根中SSuper1；2的表达降低，根和地上部中Apr的表达也降低。在硫酸盐缺乏的情况下，根对硫酸盐的吸收能力增加了3倍以上，同时伴随着根中SUltr1；1的诱导表达和SUltr1；2在根中的表达增强，以及在地上部中的SUltr1；2的诱导。硫酸盐胁迫下植株对硫酸盐吸收能力的增强、根中硫酸盐转运蛋白的表达以及根-根分配的改变，在硫酸盐胁迫下并未得到缓解，硫酸盐再补给也不会迅速影响植株的生长。缺硫植物根部和地上部APR的表达显著增强，而在H_2S暴露和硫酸盐再供应条件下，APR的表达再次降低。评价了地上部到根的相互作用以及硫酸盐和硫醇作为调节信号在SUltr1；1和1；2的活性和表达中的意义。
The activity and expression of sulfate transporters and adenosine 5'-phosphosulfate (APS) reductase (APR) in plants are modulated by the plant sulfur status and the demand for growth. To elucidate regulatory mechanisms in Chinese cabbage [Brassica pekinensis (Lour.) Rupr.], the interactions between atmospheric H2S and sulfate nutrition and the impact on the activity and expression of the Group I sulfate transporters and APR were studied. At an ample sulfate supply, H2S exposure of Chinese cabbage resulted in a partial decrease of the sulfate uptake capacity, and at concentrations >= 0.25 mu L L-1 a decreased expression of Sultr1;2 in the root and APR in the root and shoot. Upon sulfate deprivation there was a more than 3-fold increase in the sulfate uptake capacity of the root, accompanied by an induced expression of Sultr1;1 and an enhanced expression of Sultr1;2 in the root, along with an induction of Sultr1;2 in the shoot. The enhanced sulfate uptake capacity, the expression of the sulfate transporters in the root and the altered shoot-to-root partitioning appearing during sulfate deprivation were not alleviated upon H2S exposure and not rapidly affected by sulfate re-supply. Expression of APR was strongly enhanced in the root and shoot of sulfate-deprived plants and decreased again upon H2S exposure and sulfate re-supply. The significance of shoot-to-root interaction and sulfate and thiols as regulating signals in the activity and expression of Sultr1;1 and 1;2 is evaluated.