Simple fluorescent pyrimidine analogues detect the presence of DNA abasic sites
Simple fluorescent pyrimidine analogues detect the presence of DNA abasic sites
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DOI：
10.1021/ja052000a
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发表时间：
2005-08-10
影响因子：
15
通讯作者：
Tor, Y
中科院分区：
文献类型：
--
作者：
Greco, NJ;Tor, Y
合成了一类简单的嘧啶类似物，并研究了它们的物理化学性质。家族中最敏感的基因被整合到DNA中。这种电子等排荧光DNA类似物通过荧光监测DNA双链体的变性，并阳性检测DNA双链体中脱碱基位点的存在。
A family of simple pyrimidine analogues has been synthesized, and their photophysical properties have been investigated. The most responsive of the family was incorporated in DNA. This isosteric fluorescent DNA analogue monitors denaturation of a DNA duplex via fluorescence and positively detects the presence of abasic sites in DNA duplexes.