A novel reaction force-fluorescence measurement system for evaluating pancreatic juice leakage from an excised swine pancreas during distal pancreatectomy.
A novel reaction force-fluorescence measurement system for evaluating pancreatic juice leakage from an excised swine pancreas during distal pancreatectomy.
复制标题
一种新型反作用力荧光测量系统，用于评估远端胰腺切除术期间离体猪胰腺的胰液渗漏。
DOI：
10.1002/jhbp.775
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
D Kim.
中科院分区：
文献类型：
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作者：
E Kobayashi,S Tsuchiya,Y Akagi,N Tomii,K Nakagawa;K Inai;Y Muragaki;T Asano;D Kim.
背景：吻合器切除术是胰远端切除术的常用方法。然而，由此导致的胰液泄漏是一个严重的问题。我们开发了一种力荧光测量方法，作为定量评估组织在压迫下撕裂引起的胰腺渗漏的第一步。方法该系统包括一个大小与订书机相似的压头测试机，用于控制压缩速度和测量反作用力；一个荧光测量系统用于测量胰液泄漏。胰液漏量以荧光强度增加速率的最大值（max value）来测量。10个切除的猪胰腺分别以500、100、10 mm/min的速度压缩至2 mm厚度。结果压缩前后最大值的增加与组织破坏引起的反作用力下降量呈强正相关（0.804）。缓慢压缩（10 mm/min）时未见胰液渗漏。结论我们成功地开发了一种新的力荧光测量系统，可以检测和定量组织撕裂引起的胰液泄漏。该系统可以确定防止胰液泄漏的最佳压缩条件。
BackgroundResection using a stapler is a popular approach to distal pancreatectomy. However, the resulting leakage of pancreatic juice represents a serious problem. We have developed a force‐fluorescence measurement as a first step towards the quantitative evaluation of pancreatic leakage due to tissue tearing under compression.MethodsThe system comprises a testing machine with an indenter, similar in size to a stapler, which controls compression speed and measures reaction force, and a fluorescence measurement system to measure pancreatic juice leakage. Pancreatic juice leakage is measured as the maximum value of the increasing rate of fluorescence intensity (max value). Ten excised swine pancreases were compressed at a speed of 500, 100, and 10 mm/min until their thicknesses became 2 mm.ResultsA strong positive correlation (0.804) was observed between the increase in max value before and after compression and the amount of reaction force drop due to tissue destruction. No pancreatic juice leakage was observed when compressed slowly (10 mm/min).ConclusionsWe have successfully developed a novel force‐fluorescence measurement system that can detect and quantify pancreatic juice leakage caused by tissue tearing. This system can determine the optimal compression conditions for preventing pancreatic juice leakage.