DEVELOPMENTAL EXPRESSION OF AN ANDROGEN-REGULATED EPIDIDYMAL PROTEIN
DEVELOPMENTAL EXPRESSION OF AN ANDROGEN-REGULATED EPIDIDYMAL PROTEIN
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DOI：
10.1210/endo-125-2-942
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发表时间：
1989-08-01
期刊：
影响因子：
4.8
通讯作者：
FRENCH, FS
中科院分区：
文献类型：
--
作者：
CHAREST, NJ;PETRUSZ, P;FRENCH, FS
采用RIA法测定了成年大鼠附睾提取物中雄激素调节的大鼠附睾分泌蛋白酸性附睾糖蛋白（AEG）的含量。虽然在1日龄时可检测到AEG，但直到20日龄时才检测到显著的AEG浓度；浓度稳步增加，到120日龄时，AEG占总可溶性蛋白的10%。对5日龄动物附睾的RNA进行Northern blot检测，AEG mRNA在20 ~ 35日龄期间迅速增加，在45日龄时达到最大值。利用免疫组化技术，AEG定位于1日龄的附睾上皮细胞。15 d后AEG染色细胞数量明显增加。在第120天，免疫反应性主要局限于上皮管的管腔。为了描述可能影响发育中的附睾AEG表达的因素，我们测量了不同年龄动物组织提取物中雄激素和雄激素受体mRNA的浓度。在1 ~ 90日龄动物的附睾提取物中检测到雄激素受体mRNA。所有年龄段的附睾雄激素浓度均较高（范围为6.0 ~ 31.2 ng/g）。20日龄时AEG mRNA浓度的显著增加与雄激素或雄激素受体mRNA浓度的增加无关，表明AEG表达可能需要其他因素。
Acidic epididymal glycoprotein (AEG), an androgen-regulated secretory protein of rat epididymis, was quantitated by RIA in epididymal extracts of rats of increasing age. Although detectable at 1 day of age, significant concentrations of AEG were not measured until 20 days; concentrations increased steadily, so that by 120 days of age, AEG represented 10% of total soluble protein. AEG mRNA was detected by Northern blot analysis of RNA from epididymides of 5-day-old animals and rapidly increased in amount between 20 and 35 days, reaching a maximum at 45 days. Using immunohistochemistry, AEG was localized in epididymal epithelial cells at 1 day of age. The number of cells staining for AEG increased markedly after 15 days. At 120 days, the immunoreactivity was predominantly localized to the lumen of the epithelial duct. To delineate factors that may influence AEG expression in the developing epididymis, we measured concentrations of androgen and androgen receptor mRNA in tissue extracts prepared from animals of various ages. Androgen receptor mRNA was detectable in epididymal extracts isolated from 1- to 90-day-old animals. Epididymal androgen concentrations were high at all ages (range, 6.0-31.2 ng/g tissue). The marked increase in AEG mRNA concentration at 20 days of age was not associated with an increase in either androgen or androgen receptor mRNA concentrations, suggesting that other factors may be necessary for AEG expression.