Resveratrol Alters microRNA Expression Profiles in A549 Human Non-Small Cell Lung Cancer Cells
Resveratrol Alters microRNA Expression Profiles in A549 Human Non-Small Cell Lung Cancer Cells
复制标题
DOI：
10.1007/s10059-011-1037-z
复制
发表时间：
2011-09-01
影响因子：
3.8
通讯作者：
An, Sungkwan
中科院分区：
文献类型：
--
作者：
Bae, Seunghee;Lee, Eun-Mee;An, Sungkwan
白藜芦醇是一种植物酚类植物抗毒素，据报道在几种癌症中具有抗肿瘤特性。特别是，一些研究表明白藜芦醇对A549人非小细胞肺癌细胞具有抗增殖作用；然而，其作用机制仍不完全清楚。microRNAs （miRNAs）是一类参与基因表达的小的、非编码的、调节的RNA分子，其解除调控与肺癌密切相关。在这项研究中，我们证明了白藜芦醇处理改变了A549细胞中miRNA的表达。通过微阵列分析，我们鉴定出71个mirna在白藜芦醇处理的细胞中相对于未处理细胞中的表达水平表现出2倍以上的表达变化。此外，我们利用miRNA靶标预测程序确定了与细胞凋亡、细胞周期调节、细胞增殖和分化相关的靶基因。总之，我们的数据表明，白藜芦醇诱导了A549细胞miRNA表达谱的显著变化，为研究白藜芦醇的抗癌机制提供了一种新的方法。
Resveratrol is a plant phenolic phytoalexin that has been reported to have antitumor properties in several types of cancers. In particular, several studies have suggested that resveratrol exerts antiproliferative effects against A549 human non-small cell lung cancer cells; however, its mechanism of action remains incompletely understood. Deregulation of microRNAs (miRNAs), a class of small, noncoding, regulatory RNA molecules involved in gene expression, is strongly correlated with lung cancer. In this study, we demonstrated that resveratrol treatment altered miRNA expression in A549 cells. Using microarray analysis, we identified 71 miRNAs exhibiting greater than 2-fold expression changes in resveratrol-treated cells relative to their expression levels in untreated cells. Furthermore, we identified target genes related to apoptosis, cell cycle regulation, cell proliferation, and differentiation using a miRNA target-prediction program. In conclusion, our data demonstrate that resveratrol induces considerable changes in the miRNA expression profiles of A549 cells, suggesting a novel approach for studying the anticancer mechanisms of resveratrol.