Particulate matter air pollution stimulates monocyte release from the bone marrow
Particulate matter air pollution stimulates monocyte release from the bone marrow
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DOI：
10.1164/rccm.200402-235oc
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发表时间：
2004-10-15
影响因子：
24.7
通讯作者：
van Eeden, SF
中科院分区：
文献类型：
--
作者：
Goto, Y;Ishii, H;van Eeden, SF
颗粒空气污染（PM10）刺激肺泡巨噬细胞（AM）从骨髓（BM）释放未成熟的粒细胞进入循环。本研究旨在确定PM10（环境EHC-93或惰性碳[CC]）滴注暴露对BM中单核细胞释放的影响以及AM在该反应中的作用。通过静脉注射5 ′-溴-2 ′-脱氧尿苷在体内标记兔骨髓中的单核细胞前体，并通过将颗粒或暴露于颗粒的AM上清液滴入肺中来测定PM 10的作用。滴注EHC-93（500 μ g/ml）或来自与EHC-93孵育的AM的上清液（100 μ g/ml）循环带状细胞计数增加（p < 0.05）并缩短单核细胞通过BM的通过时间（35.5 +/- 2.2至25.0 +/- 1.5小时或36.2 +/- 2.6至25.7 +/- 1.8小时，p < 0.05）。CC（1%）滴注也使单核细胞BM通过时间缩短至28.4 +/-1.9小时（p < 0.05），但与CC孵育的AM上清液没有缩短。我们的结论是，暴露于大气PM10刺激AM产生介质，而这些细胞因子加速单核细胞从BM中释放。
Particulate air pollution (PM10) stimulates alveolar macrophages (AMs) to release immature granulocytes from the bone marrow (BM) into the circulation. This study was designed to determine the effect of PM10 (ambient EHC-93 or inert carbon [CC]) instillation exposure on the monocyte release from the BM and the role of AM in this response. Monocyte precursors were labeled in the BM of rabbits in vivo by an intravenous injection of 5'-bromo-2'-deoxyuridine, and the effects of PM10 were determined by instillation either particles or supernatants of AM exposed to particles into the lungs. Instillation of EHC-93 (500 mug/ml) or supernatants from AM incubated with EHC-93 (100 mug/ml) increased circulating band cell counts (p < 0.05) and shortened the transit time of monocytes through the BM (35.5 +/- 2.2 to 25.0 +/- 1.5 hours or 36.2 +/- 2.6 to 25.7 +/- 1.8 hours, p < 0.05) compared with the control subject. CC (1%) instillation also shortened the monocyte BM transit time to 28.4 +/- 1.9 hours (p < 0.05), but supernatants of AM incubated with CC did not. We conclude that exposure to atmospheric PM10 stimulates the production of mediators by AM, and these cytokines accelerate the monocyte release from the BM.