Gemini Surfactant-Protein Interactions: Effect of pH, Temperature, and Surfactant Stereochemistry
Gemini Surfactant-Protein Interactions: Effect of pH, Temperature, and Surfactant Stereochemistry
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DOI：
10.1021/bm9004723
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发表时间：
2009-09-01
影响因子：
6.2
通讯作者：
Garcia-Rio, Luis
中科院分区：
文献类型：
--
作者：
Faustino, Celia M. C.;Calado, Antonio R. T.;Garcia-Rio, Luis
研究了胱氨酸衍生的双子表面活性剂与牛血清白蛋白（BSA）的相互作用，并与传统的半胱氨酸衍生的单链表面活性剂进行了比较。研究了Gemini表面活性剂的立体化学对BSA的吸附行为的影响，以及pH和温度对吸附行为的影响。电导率和表面张力测量用于获得重要的系统参数，如临界聚集浓度（cac），聚合物饱和点（psp），电离度（α），和表面活性剂结合蛋白质的量（M）。立体化学被发现会影响所研究的表面活性剂的表面性质和它们与BSA的相互作用，但不会影响它们在溶液中的胶束性质。
The interactions between bovine serum albumin (BSA) and gemini surfactants derived from cystine have been investigated and were compared with the conventional single-chain surfactant derived from cysteine. The influence of the stereochemistry of the gemini surfactant on its behavior toward BSA was also investigated, as well as the effects of pH and temperature. Electrical conductivity and surface tension measurements were used to obtain important system parameters such as critical aggregation concentration (cac), polymer saturation point (psp), degree of ionization (alpha), and the amount of surfactant binding to protein (M). Stereochemistry was found to influence the surface properties of the surfactants studied and their interaction with BSA but not their micellar properties in solution.