FGF signaling regulates expression of Tbx2, Erm, Pea3, and Pax3 in the early nasal region
FGF signaling regulates expression of Tbx2, Erm, Pea3, and Pax3 in the early nasal region
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DOI：
10.1006/dbio.2002.0696
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发表时间：
2002-07-15
影响因子：
2.7
通讯作者：
Neubüser, A
中科院分区：
文献类型：
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作者：
Firnberg, N;Neubüser, A
Fgf8是鼻腔区域正常发育所必需的。在这里，我们使用了一种候选方法来鉴定鸡鼻间充质响应FGF信号而诱导的基因。通过外植体培养系统，我们发现转录因子Tbx2、Erm、Pea3和Pax3在鼻间质中的表达受外胚层信号以阶段依赖的方式调节，而Pax7则不受影响。通过将FGF蛋白和FGF受体拮抗剂浸泡在微球中，我们进一步证明了FGF信号对于Tbx2、Erm、Pea3和Pax3的表达是必要和充分的，但对Pax7的表达没有影响。我们还发现，在鼻腔间充质中，对FGF信号的响应能力最初是广泛而均匀的，但在发育的后期阶段仅限于正常暴露于FGF的区域，这与FGF受体表达的变化相一致。最后，我们提供的证据表明，FGF8也调节Erm和Pea3在鼻腔基板中的表达。总之，这些结果确定了Tbx2、Erm、Pea3和Pax3是面部区域FGF信号传导的下游靶点，并表明这些基因可能在鼻区发育过程中介导FGF8的一些作用。(C) 2002 Elsevier Science （USA）。
Fgf8 is required for normal development of the nasal region. Here, we have used a candidate approach to identify genes that are induced in chick nasal mesenchyme in response to FGF signaling. Using an explant culture system, we show that expression of the transcription factors Tbx2, Erm, Pea3, and Pax3, but not Pax7, in nasal mesenchyme is regulated by ectodermal signals in a stage-dependent manner. Using beads soaked in recombinant FGF protein and an FGF receptor antagonist, we furthermore demonstrate that FGF signaling is necessary and sufficient for expression of Tbx2, Erm, Pea3, and Pax3, but has no effect on Pax7 expression. We also show that, within the nasal mesenchyme, competence to respond to FGF signaling is initially widespread and uniform but becomes restricted to regions normally exposed to FGF at later stages of development, coincident with changes in FGF receptor expression. Finally, we provide evidence that FGF8 also regulates Erm and Pea3 expression in the nasal placodes. Together, these results identify Tbx2, Erm, Pea3, and Pax3 as downstream targets of FGF signaling in the facial area and suggest that these genes may mediate some of the effects of FGF8 during development of the nasal region. (C) 2002 Elsevier Science (USA).