Toxic effects of octylphenol on cultured rat and murine splenocytes.
Toxic effects of octylphenol on cultured rat and murine splenocytes.
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辛基苯酚对培养的大鼠和小鼠脾细胞的毒性作用。
DOI：
10.1006/taap.1996.0185
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Blake,CA
中科院分区：
文献类型：
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作者：
Nair-Menon,JU;Campbell,GT;Blake,CA
烷基酚聚乙氧基化物和烷基酚，例如 4-叔辛基酚 (OP)，是环境污染物。由于这些化合物对水生动物有毒，我们研究了OP对从雄性Fischer 344大鼠或雄性Balb/c小鼠中取出并进行体外培养的脾细胞的影响。培养5或27小时后通过台盼蓝排除法评估细胞活力。 5 小时后，用 0.08% ETOH（载体）或任何剂量的 OP 培养不会改变总细胞数或活细胞百分比。用两种不同的烷基酚聚乙氧基化物培养细胞 5 小时，导致所有细胞损失。培养 27 小时后，10−12mOP 或更高浓度时，活大鼠或小鼠细胞的百分比显着下降。比较 OP、地塞米松 (DEX) 和 17β-雌二醇对大鼠脾细胞的作用。培养24小时后，地塞米松比OP毒性更大； 17β-雌二醇没有毒性。地塞米松和 OP（而非 17β-雌二醇）在培养 3 小时（吖啶橙染色）或培养 4 小时（碘化丙啶染色）后引起显着的核浓缩。当小鼠脾细胞在不含 Ca2+ 的培养基中培养时，10−6mOP 的毒性被消除，但 10−6mDEX 的毒性没有消除。当细胞与 10−4 或 10−6mOP 或 10−6mDEX 一起孵育 4 小时时，通过激活的细胞分选仪分析检测到显着更多的含有游离 3'-OH DNA 末端的小鼠脾细胞。这些研究的结果表明，OP 对培养的大鼠和小鼠脾细胞具有毒性，并表明这种毒性作用至少部分是通过 Ca2+ 依赖性细胞凋亡发挥的。
Alkylphenol polyethoxylates and alkylphenols, such as 4-tert-octylphenol (OP), are environmental contaminants. Because these compounds are toxic to aquatic animals, we studied the effects of OP on splenocytes removed from male Fischer 344 rats or male Balb/c mice and culturedin vitro.Cell viability was assessed by trypan blue exclusion after 5 or 27 hr of culture. Culture with 0.08% ETOH (vehicle) or any dose of OP did not alter total cell number or the percentage of viable cells after 5 hr. Culture of cells with two different alkylphenol polyethoxylates for 5 hr resulted in the loss of all cells. The percentages of viable rat or mouse cells after 27 hr of culture were decreased significantly by 10−12mOP or greater concentrations. The actions of OP, dexamethasone (DEX), and 17β-estradiol on rat splenocytes were compared. Dexamethasone was more toxic than OP after 24 hr of culture; 17β-estradiol was not toxic. Dexamethasone and OP, but not 17β-estradiol, caused significant nuclear condensation after 3 hr of culture (acridine orange staining) or 4 hr of culture (propidium iodide staining). The toxicity of 10−6mOP, but not that of 10−6mDEX, was eliminated when mouse splenocytes were cultured in Ca2+-free medium. Significantly more mouse splenocytes containing free 3′-OH DNA ends were detected by activated cell sorter analyses when the cells had been incubated for 4 hr with 10−4or 10−6mOP or 10−6mDEX. The results of these studies demonstrate that OP is toxic to cultured rat and mouse splenocytes and suggest that this toxic effect is exerted, at least partially, through Ca2+-dependent apoptosis.