CLONING AND EXPRESSION OF A CDNA-ENCODING AN ENDOTHELIN RECEPTOR
CLONING AND EXPRESSION OF A CDNA-ENCODING AN ENDOTHELIN RECEPTOR
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DOI：
10.1038/348730a0
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发表时间：
1990-12-20
期刊：
影响因子：
64.8
通讯作者：
NAKANISHI, S
中科院分区：
文献类型：
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作者：
ARAI, H;HORI, S;NAKANISHI, S
内毒素是一个新近发现的多肽家族，由三个肽(ET-1、ET-2和ET-3)组成，它们是已知的最有效的血管收缩多肽1-3。它们在调节血管平滑肌张力1-3中起着关键作用。内皮素的不同功能被认为是通过与许多不同的受体相互作用而介导的，这些受体偶联到肌醇磷酸/钙离子信使通路。然而，由于这三种多肽在结构上的相似性，多个内皮素受体的存在和性质仍有待阐明。我们报道了编码牛内皮素受体的互补DNA的克隆，该受体具有类似于其他G蛋白偶联受体的跨膜拓扑结构，并显示出与ET-1的特异性结合，在用克隆的cDNA转染动物细胞中对ET-1具有最高的选择性。这种受体信使RNA广泛分布于中枢神经系统和周围组织，特别是心脏和肺。我们的结果支持存在其他受体亚型的观点。
ENDOTHELINS are a newly described peptide family consisting of three peptides (ET-1, ET-2 and ET-3) which are the most potent vasoconstrictive peptides known1–3. They are crucial in the regulation of vascular smooth muscle tone1–3. The diverse functions of endothelins are thought to be mediated by interaction with many different receptors coupled to the inositol phosphate/calcium ion messenger pathway. However, because of the structural resemblance of the three peptides, the presence and nature of multiple endothelin receptors remain to be elucidated. We report here the cloning of a complementary DNA encoding a bovine endothelin receptor, which has a transmembrane topology similar to that of other G protein-coupled receptors and shows specific binding, with the highest selectivity to ET-1 in animal cells trans-fected with the cloned cDNA. This receptor messenger RNA is widely distributed in the central nervous system and peripheral tissues, particularly in the heart and lung. Our results support the view that there are other receptor subtypes.