OCCURRENCE OF A COMMON LIPOPOLYSACCHARIDE ANTIGEN IN STANDARD AND CLINICAL STRAINS OF PSEUDOMONAS-AERUGINOSA
OCCURRENCE OF A COMMON LIPOPOLYSACCHARIDE ANTIGEN IN STANDARD AND CLINICAL STRAINS OF PSEUDOMONAS-AERUGINOSA
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DOI：
10.1128/jcm.27.5.962-967.1989
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发表时间：
1989-05-01
影响因子：
9.4
通讯作者：
LAM, JS
中科院分区：
文献类型：
--
作者：
LAM, MYC;MCGROARTY, EJ;LAM, JS
铜绿假单胞菌PAO 1的脂多糖（LPS）含有两种O型多糖，称为A和B带。高分子量B带LPS决定细菌的O特异性，而抗原性不同的A带LPS仅由短链多糖组成。制备了7株分泌A带特异性单克隆抗体的杂交瘤，并用于研究标准菌株和临床菌株的LPS。虽然A-带抗体不凝集任何血清型菌株目前在国际抗原分型计划，西方免疫印迹显示，11的17个血清型菌株具有A-带LPS。在一组250例囊性纤维化患者的临床分离株中，170例（68%）在凝集试验的基础上具有A带LPS，但在银染凝胶中均显示缺乏含O抗原的B带。对来自单个患者的系列分离株的调查揭示了抗原变异的模式。在感染过程中，可分型的分离株变得不可分型，并且O抗原被A带取代作为主要LPS抗原。这些结果表明，A带LPS可能是不可分型临床分离株的主要LPS抗原，也是其他铜绿假单胞菌的共同抗原。
The lipopolysaccharide (LPS) of Pseudomonas aeruginosa PAO1 contains two species of O polysaccharide termed A and B bands. The high-molecular-weight B-band LPS determines the O specificity of the bacterium, while the antigenically distinct A-band LPS consists of only shorter-chain polysaccharides. Seven hybridomas secreting A-band specific monoclonal antibodies were produced and used to study the LPS of standard and clinical strains. Although A-band antibodies did not agglutinate any of the serotype strains presently in the International Antigenic Typing Scheme, Western immunoblots revealed that 11 of the 17 serotype strains possessed A-band LPS. In a group of 250 clinical isolates from patients with cystic fibrosis, 170 (68%) had A-band LPS on the basis of agglutination tests, but in silver-stained gels all were shown to be deficient in O-antigen-containing B band. Investigation of serial isolates from a single patient revealed a pattern of antigenic variation. During the course of the infection, serotypeable isolates became nontypeable, and the O antigen was replaced with A band as the major LPS antigen. These results suggest that A-band LPS may be the major LPS antigen in nontypeable clinical isolates and a common antigem among other P. aeruginosa strains.