SCF and IL-31 rather than IL-17 and BAFF are potential indicators in patients with allergic asthma
SCF and IL-31 rather than IL-17 and BAFF are potential indicators in patients with allergic asthma
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DOI：
10.1111/j.1398-9995.2007.01566.x
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发表时间：
2008-03-01
期刊：
影响因子：
12.4
通讯作者：
Huang, B.
中科院分区：
文献类型：
--
作者：
Lei, Z.;Liu, G.;Huang, B.
背景：尽管过敏性哮喘的患病率在过去十年中迅速增加，但其诊断标准尚未得到很好的建立。本研究的目的是探讨是否干细胞因子（SCF），B细胞活化因子（BAFF），和细胞因子白细胞介素（IL）-17和IL-31是有用的参数诊断过敏性asthmatic.Methods：血液样本收集过敏性哮喘患者，对照组患者和健康对照组。采用酶联免疫吸附法测定血清SCF、BAFF、IL-17和IL-31浓度。结果：SCF和IL-31蛋白表达水平与mRNA表达水平之间存在良好的相关性（SCF：r = 0.6162; IL-31：r = 0.5463）。过敏性哮喘患者血清SCF和IL-31浓度均显著高于正常对照组（SCF：中位数1.83vs0.85ng/ml，P < 0.01; IL-31：中位数50.15vs10.01pg/ml，P < 0.001）。过敏性哮喘患者外周血单个核细胞中SCF和IL-31 mRNA水平也明显高于正常对照组（分别为P = 0.002和P < 0.001）。这些发现表明，过敏性哮喘的特征是细胞因子SCF和IL-1升高。31的蛋白水平和PBMC中mRNA水平的表达水平的测定将是诊断过敏性哮喘的一个有价值的参数。
Background: Although the prevalence of allergic asthma increased quickly in the past decade, the diagnostic criteria have not been well established. The aim of the present study was to explore whether stem cell factor (SCF), B cell-activating factor (BAFF), and cytokines interleukin (IL)-17 and IL-31 are usable parameters for the diagnosis of allergic asthmatics.Methods: Blood samples were collected from patients with allergic asthma, control patients, and healthy control subjects. The serum concentrations of SCF, BAFF, IL-17, and IL-31 were measured by enzyme-linked immunosorbent assay. The corresponding mRNA levels in peripheral blood mononuclear cells (PBMCs) were determined by real-time reverse-transcription polymerase chain reaction.Results: A good correlation existed between protein levels of SCF and IL-31 and their mRNA levels (SCF: r = 0.6162; IL-31: r = 0.5463). The serum concentrations of SCF and IL-31 in allergic asthmatic patients, but not control patients, were significantly higher than those in normal control subjects (SCF: median 1.83 vs 0.85 ng/ml, P < 0.01; IL-31: 50.15 vs 10.01 pg/ml, P < 0.001). Consistently, the levels of SCF and IL-31 mRNAs in allergic asthmatic patients' PBMCs were also significantly higher than those in normal control subjects (P = 0.002 and P < 0.001, respectively).Conclusions: These findings suggest that allergic asthma is characterized by an elevation of cytokines SCF and IL-31 and the measurement of their expression at either protein level in serum or mRNA level in PBMCs will be a valuable parameter for the diagnosis of allergic asthma.