DETECTION OF VARICELLA-ZOSTER VIRUS-DNA IN AIR SAMPLES FROM HOSPITAL ROOMS
DETECTION OF VARICELLA-ZOSTER VIRUS-DNA IN AIR SAMPLES FROM HOSPITAL ROOMS
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DOI：
10.1093/infdis/169.1.91
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发表时间：
1994-01-01
影响因子：
6.4
通讯作者：
WALLACE, MR
中科院分区：
文献类型：
--
作者：
SAWYER, MH;CHAMBERLIN, CJ;WALLACE, MR
水痘带状疱疹病毒（VZV）是一种高度传染性的传染性病原体，可在机构环境中引起疫情。VZV的传播被认为发生在与受感染个体直接接触和通过气溶胶传播之后。为了记录VZV的雾化作用，采用聚合酶链反应（PCR）方法检测从活动性VZV感染患者病房采集的空气样本中的VZV DNA。来自活动性水痘患者房间的78份空气样本中有64份（82%）检测到VZV DNA，来自带状疱疹患者房间的13份样本中有9份（70%）检测到VZV DNA。VZV在离患者床1.2-5.5 m处和皮疹出现后1-6天内检测到。在某些情况下，可以在医院隔离病房外检测到VZV DNA。这种基于pcr的方法允许VZV雾化的检测和半定量，可以成为监测工作的有用工具，以控制环境中的VZV气溶胶。
Varicella-zoster virus (VZV) is a highly contagious infectious agent that causes outbreaks in institutional settings. Transmission of VZV is felt to occur following direct contact with an infected individual and by aerosol spread. To document the aerosolization of VZV, a polymerase chain reaction (PCR) assay was used to detect VZV DNA in air samples obtained from hospital rooms of patients with active VZV infection. VZV DNA was detected in 64 (82%) of 78 air samples from rooms housing patients with active varicella and 9 (70%) of 13 samples from rooms of patients with herpes zoster. VZV was detected 1.2-5.5 m from patients' beds and for 1-6 days following onset of rash. On some occasions, VZV DNA could be detected outside the hospital isolation rooms housing patients. This PCR-based method allows the detection and semiquantitation of VZV aerosolization and can be a useful tool for monitoring efforts to control VZV aerosols in the environment.