Development of porcine embryos produced by IVM/IVF in a medium with or without protein supplementation: effects of extracellular glutathione
Development of porcine embryos produced by IVM/IVF in a medium with or without protein supplementation: effects of extracellular glutathione
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在添加或不添加蛋白质的培养基中通过 IVM/IVF 产生的猪胚胎的发育：细胞外谷胱甘肽的影响
DOI：
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
B. N. Day
中科院分区：
文献类型：
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作者：
Wei‐Hua Wang;B. N. Day
本研究旨在研究细胞外还原型谷胱甘肽对猪体外成熟（IVM）和体外受精（IVF）胚胎发育的影响，这些胚胎在化学成分确定的北卡罗来纳州州立大学（NCSU）23培养基或在NCSU 23培养基中与牛血清白蛋白（BSA）。微丝分布，作为胚胎质量的标志，也通过免疫细胞化学染色和共聚焦显微镜检查。当受精卵母细胞在含有0-0.5 mM谷胱甘肽的限定培养基中培养时，仅在含有谷胱甘肽的培养基中观察到囊胚形成（8.5-16.0%）。随着谷胱甘肽浓度的增加，囊胚中的卵裂球数量增加（18.8±7.2至31.0±8.6）。在含有4 mg BSA/ml的NCSU 23培养基中，与对照组（29.5%）相比，添加0.125-0.5 mM谷胱甘肽显著增加了发育成囊胚的卵母细胞比例（39.2-52.5%）。然而，在囊胚中的平均细胞数方面没有观察到差异（41.9±15.6至49.1±15.5）。在限定培养基和含BSA的培养基中产生的胚胎中微丝分布没有显著差异。这些结果表明，猪IVM/IVF生产的胚胎可以在限定的培养基中发育到囊胚期。BSA和谷胱甘肽对猪胚胎发育有协同作用。
This study was designed to examine the effects of extracellular reduced glutathione on development of pig embryos, produced by in vitro maturation (IVM) and in vitro fertilisation (IVF), in a chemically defined North Carolina State University (NCSU) 23 medium or in NCSU 23 medium with bovine serum albumin (BSA). Microfilament distribution, as a marker of embryo quality, was also examined by immunocytochemical staining and confocal microscopy. When the inseminated oocytes were cultured in the defined medium containing 0-0.5 mM glutathione, blastocyst formation as observed only in the media with glutathione (8.5-16.0%). Increased numbers of blastomeres were observed in the blastocysts as the concentration of glutathione was increased (18.8±7.2 to 31.0±8.6). In NCSU 23 medium with 4 mg BSA/ml, addition of glutathione at concentrations of 0.125-0.5 mM significantly increased the proportions of oocytes that developed to blastocysts (39.2-52.5%) compared with the control (29.5%). However, no difference was observed in the average cell number in the blastocysts (41.9±15.6 to 49.1±15.5). There were no significant differences in the microfilament distribution in the embryos produced in the defined medium and in the BSA-containing medium. These results indicate that pig embryos produced by IVM/IVF can develop to the blastocyst stage in a defined medium. BSA and glutathione have a synergistic effect on pig embryo development.
影响因子：
3.6
作者：
Gardiner, CS;Salmen, JJ;Stover, SK
通讯作者：
Stover, SK
影响因子：
3.6
作者：
GARDINER, CS;REED, DJ
通讯作者：
REED, DJ
影响因子：
2.8
作者：
Abeydeera, LR;Wang, WH;Day, BN
通讯作者：
Day, BN