Modulation of testicular macrophage activity by collagenase.
Modulation of testicular macrophage activity by collagenase.
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DOI：
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发表时间：
2005
影响因子：
1.5
通讯作者：
K. Bryniarski;M. Szczepanik;M. Ptak;W. Ptak
中科院分区：
文献类型：
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作者：
K. Bryniarski;M. Szczepanik;M. Ptak;W. Ptak
睾丸巨噬细胞（Testicular macrophages，TM）位于男性性腺的间质组织中。这些吞噬细胞参与形成器官特异性功能性血睾屏障，并参与调节局部激素平衡。在本研究中，我们使用先前描述的方法-机械（M-TM）或酶促，通过用胶原酶（E-TM）处理从睾丸组织中分离TM，然后我们研究了这些细胞产生的几种细胞因子和活性氧中间体（ROI）。将类似处理的油诱导的腹膜巨噬细胞（PM）用作对照细胞。PM具有比M-TM更高的TNF-α、IL-6、IL-10和IL-12产生的基线水平，并且胶原酶处理增加了两种细胞群的这些细胞因子（IL-12除外）的产生。这种效应在TM中显著表达更多。与PM相反，TM在酵母多糖刺激下产生的ROI很小。我们的结论是，在睾丸局部炎症的情况下，ROI阴性的TM不会导致组织损伤，而是可能将局部免疫反应导向体液途径。
Testicular macrophages (TMs) are located in the interstitial tissue of male gonad. These phagocytic cells take part in forming the organ-specific functional blood-testis barrier and participate in the regulation of the local hormonal balance. In the present study, we isolated TMs from testicular tissues using previously described methods--mechanical (M-TMs) or enzymatic, by treatment with collagenase (E-TMs) and then we studied production by these cells of several cytokines and reactive oxygen intermediates (ROI's). Similarly treated oil-induced peritoneal macrophages (PMs) were used as control cells. PMs had a higher baseline level of production of TNF-alpha, IL-6, IL-10 and IL-12 than M-TMs and collagenase treatment increased the production of these cytokines (except IL-12) by both cell populations. This effect was significantly more expressed in TMs. In contrast to PMs, TMs produced little ROI's when stimulated by zymosan. We conclude that in the case of local inflammation in the testis, ROI-negative TMs do not contribute to the tissue damage and instead may direct the local immune response into humoral pathway.