pygopus 2 has a crucial, Wnt pathway-independent function in lens induction
pygopus 2 has a crucial, Wnt pathway-independent function in lens induction
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DOI：
10.1242/dev.001495
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发表时间：
2007-05-15
期刊：
影响因子：
4.6
通讯作者：
Lang, Richard A.
中科院分区：
文献类型：
--
作者：
Song, Ni;Schwab, Kristopher R.;Lang, Richard A.
果蝇Pygopus最初被鉴定为经典Wnt信号通路的核心组分和转录共激活因子。在这里，我们研究了小眼球，出现在小鼠与生殖系无效突变的pygopus 2。我们表明，这种表型是一个有缺陷的透镜发展在诱导阶段的后果。使用一系列区域有限的Cre重组酶转基因的Pygo2（flox）的条件性缺失，我们表明，Pygo2活性在前基板推定透镜外胚层，基板外胚层和眼间充质都有助于透镜的发展。在每种情况下，Pygo2都是关键转录因子Pax6正常表达所必需的。最后，我们提供了多条证据表明，虽然Pygo2可以在Wnt通路中发挥作用，但其在透镜发育中的活性是Wnt通路独立的。
Drosophila Pygopus was originally identified as a core component of the canonical Wnt signaling pathway and a transcriptional coactivator. Here we have investigated the microophthalmia that arises in mice with a germline null mutation of pygopus 2. We show that this phenotype is a consequence of defective lens development at inductive stages. Using a series of regionally limited Cre recombinase transgenes for conditional deletion of Pygo2(flox), we show that Pygo2 activity in pre-placodal presumptive lens ectoderm, placodal ectoderm and ocular mesenchyme all contribute to lens development. In each case, Pygo2 is required for normal expression levels of the crucial transcription factor Pax6. Finally, we provide multiple lines of evidence that although Pygo2 can function in the Wnt pathway, its activity in lens development is Wnt pathway-independent.