Fabrication of Stand-Alone Cell-Laden Collagen Vascular Network Scaffolds Using Fugitive Pattern-Based Printing-Then-Casting Approach
Fabrication of Stand-Alone Cell-Laden Collagen Vascular Network Scaffolds Using Fugitive Pattern-Based Printing-Then-Casting Approach
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DOI：
10.1021/acsami.8b09177
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发表时间：
2018-08-29
影响因子：
9.5
通讯作者：
Huang, Yong
中科院分区：
文献类型：
--
作者：
Jin, Yifei;Chai, Wenxuan;Huang, Yong
血管网络在组织工程中具有重要意义，是构建人体组织的第一步。尽管已经研究了各种技术来产生血管和血管样网络，但由于纯胶原溶液的可挤出性差、机械性能弱和交联时间长，因此基于独立纯胶原的血管构建体的制造仍然是一个挑战。在这项研究中，一个短暂的模式为基础的印刷然后铸造的方法进行了研究。所提出的基于藻酸盐的短效油墨具有优异的机械强度（通过添加Laponite纳米粘土）、可印刷性（通过添加Laponite纳米粘土）和可控的凝胶化速率（通过添加磷酸氢二钠）。使用这种短效油墨，复杂的脉管状结构可以容易地在Laponite EP浴中印刷和交联作为短效脉管树图案。然后将每个易逝的血管树图案嵌入明胶浴中以制作具有树图案的明胶模具。在柠檬酸钠的帮助下，易逝的血管树图案被液化并移除，以创建具有血管通道的明胶模具。最后，通过将载有细胞的胶原悬浮液浇铸到明胶模具中并在37 ℃下将其从模具中释放，可以制造嵌入成纤维细胞的独立胶原血管网络支架。细胞相关的研究表明，细胞在纯胶原血管网支架上生长和铺展良好。所提出的混合印刷然后浇铸方法也提供了一种可行的技术，用于制造具有低粘度、长凝胶化时间和较差机械性能的材料。
Vascular networks are of great significance in tissue engineering and viewed as the first step to fabricate human tissues. Although various techniques have been investigated to create vascular and vascular-like networks, the fabrication of stand-alone pure collagen based vascular constructs is still a challenge because of the poor extrudability, weak mechanical property, and long cross-linking time of pure collagen solutions. In this study, a fugitive pattern-based printing then-casting approach is investigated. The proposed alginate-based fugitive ink has excellent mechanical strength (by adding Laponite nanoclay), printability (by adding Laponite nanoclay), and controllable gelation rate (by adding disodium hydrogen phosphate). Using this fugitive ink, complex vascular-like structures can be easily printed and cross-linked in Laponite EP bath as fugitive vascular tree patterns. Each fugitive vascular tree pattern is then embedded in a gelatin bath to make a gelatin mold with the tree patterns. With the help of sodium citrate, the fugitive vascular tree pattern is liquefied and removed to create the gelatin mold with vascular channels. Finally, a stand-alone collagen vascular network scaffold embedded with fibroblasts can be fabricated by casting the cell-laden collagen suspension into the gelatin mold and releasing it from the mold at 37 degrees C. The cell-related investigations indicate that the cells grow and spread well in the pure collagen vascular network scaffold. The proposed hybrid printing-then-casting approach also provides a feasible technology to fabricate with materials having low viscosity, long gelation time, and poor mechanical property.