Viruses in Horses with Neurologic and Respiratory Diseases
Viruses in Horses with Neurologic and Respiratory Diseases
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DOI：
10.3390/v11100942
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发表时间：
2019-10-01
期刊：
影响因子：
4.7
通讯作者：
Delwart, Eric
中科院分区：
文献类型：
--
作者：
Altan, Eda;Li, Yanpeng;Delwart, Eric
宏基因组学用于鉴定13匹具有不明原因的神经系统体征的马的血浆和CSF（脑脊髓液）中的病毒序列以及14匹具有不明原因的呼吸系统体征的马的血浆和呼吸道拭子中的病毒序列。在神经系统病例的血浆中检测到马肝炎病毒和两种副细小病毒（马细小病毒-CSF和一种新型细小病毒）。来自具有呼吸道体征的马的血浆含有相同的两种副细小病毒加马细小病毒D，呼吸道拭子含有马疱疹病毒2和5。基于遗传距离，新的copiparvovirus资格作为一个新的细小病毒物种的成员，我们命名为Eqcopivirus。通过实时PCR检测这些样本和另外41份来自健康马的血浆样本中的多种马细小病毒和肝炎病毒。超过一半的样本检测呈阳性的一至三个病毒与eqcopivirus DNA检测20.5%，马肝炎病毒RNA和马细小病毒-H DNA各16%，马细小病毒-CSF DNA在12%的马。比较血浆中的病毒流行率，在这次有限的采样中，目前三种具有遗传特征的马细小病毒（均属于辅细小病毒属）均与神经系统和呼吸道体征显着相关。
Metagenomics was used to identify viral sequences in the plasma and CSF (cerobrospinal fluid) of 13 horses with unexplained neurological signs and in the plasma and respiratory swabs of 14 horses with unexplained respiratory signs. Equine hepacivirus and two copiparvoviruses (horse parvovirus-CSF and a novel parvovirus) were detected in plasma from neurological cases. Plasma from horses with respiratory signs contained the same two copiparvoviruses plus equine pegivirus D and respiratory swabs contained equine herpes virus 2 and 5. Based on genetic distances the novel copiparvovirus qualified as a member of a new parvovirus species we named Eqcopivirus. These samples plus another 41 plasma samples from healthy horses were tested by real-time PCRs for multiple equine parvoviruses and hepacivirus. Over half the samples tested were positive for one to three viruses with eqcopivirus DNA detected in 20.5%, equine hepacivirus RNA and equine parvovirus-H DNA in 16% each, and horse parvovirus-CSF DNA in 12% of horses. Comparing viral prevalence in plasma none of the now three genetically characterized equine parvoviruses (all in the copiparvovirus genus) was significantly associated with neurological and respiratory signs in this limited sampling.