Purification of silver and gold nanoparticles from two species of brown seaweeds (Padina tetrastromatica and Turbinaria ornata)
Purification of silver and gold nanoparticles from two species of brown seaweeds (Padina tetrastromatica and Turbinaria ornata)
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从两种棕色海藻（Padina tetratromatica 和 Turbinaria ornata）中纯化银和金纳米颗粒
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
K. Kathiresan
中科院分区：
文献类型：
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作者：
K. Kayalvizhi;N. Asmathunisha;V. Subramanian;K. Kathiresan
本文研究了两种棕色海藻（Padina tetrastromatica和Turbinaria ornata）合成纳米银和纳米金的分析方法及其抗菌活性。采集Mandabam海域的褐藻、四星藻和褐藻，将采集到的新鲜海藻用蒸馏水彻底清洗。用蒸馏水称约20克海藻的重量。这是在100摄氏度的热板中煮的。在水达到沸点后，得到淡黄色的海藻提取物，保存在干净无菌的螺旋管中供进一步研究。通过肉眼观察和紫外-可见吸收光谱证实了银和金纳米颗粒的显色性。通过扫描电子显微镜和动态光散射测定纳米颗粒的大小。通过FTIR确定了可能的合成基团。观察了银和金纳米颗粒对人类病原体的抗菌活性。纳米银呈褐色，纳米金呈红宝石红色，经紫外可见分光光度计进一步证实。扫描电子显微镜和动态光散射分析显示，银和金的颗粒尺寸分别为18 ~ 90 nm和20 ~ 90 nm，呈立方体形状。银和金纳米粒子似乎与羟基和羰基等化学物质有关，可能是芳香醇和胺。纳米颗粒的抗菌活性比抗真菌活性更明显。本研究提出了一种以褐藻提取物为还原剂，快速、简便、高效地合成抗菌银、金纳米粒子的方法。
The present study investigated the synthesis of silver and gold nanoparticles by two species of brown seaweeds (Padina tetrastromatica and Turbinaria ornata) through analytical techniques and its antimicrobial activity. The brown seaweeds, P. tetrastromatica and T. ornata were collected from Mandabam, The collected freshly seaweeds were thoroughly washed in distilled water. About 20 grams of seaweeds were weighed with distilled water. This was boiled in hot plate at 100 0C. After reaching the boiling point of the water, the seaweed extracts were obtained in yellowish and it was stored in clean and sterile screw tubes for further studies. The color formation of silver and gold nanoparticles was confirmed by visual observation and UV-Vis absorption spectrum. The size of the nanoparticles was determined by scanning electron microscopy and dynamic light scattering. The possible group responsible for the synthesis was confirmed by FTIR. Silver and gold nanoparticles were observed for antimicrobial activity against human pathogens. Appearance of brown and ruby red color was an indication of silver and gold nanoparticles respectively and it was further confirmed by UV-Vis spectrophotometer. Scanning electron microscopy and Dynamic light scattering analysis revealed the particles with cubical shape with size of 18-90 nm for silver and 20-90 nm for gold. The silver and gold nanoparticles appeared to be associated with chemicals like as hydroxyl and carbonyl groups, probably aromatic alcohols and amines. The antibacterial activity of nanoparticles was more pronounced than antifungal activity. The work suggested a quick, easy and efficient synthesis of antimicrobial silver and gold nanoparticles by brown seaweed extracts as reducing agents.