Modification of photosynthetic regulation in tomato overexpressing glutathione peroxidase
Modification of photosynthetic regulation in tomato overexpressing glutathione peroxidase
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DOI：
10.1016/j.bbagen.2005.04.018
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发表时间：
2005-06-20
影响因子：
3
通讯作者：
Roeckel-Drevet, P
中科院分区：
文献类型：
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作者：
Herbette, S;Le Menn, A;Roeckel-Drevet, P
为了研究谷胱甘肽过氧化物酶（GPX）在植物中的功能，我们制备了过量表达真核硒非依赖性GPX（GPX 5）的转基因番茄植株。我们在这里表明，总GPX活性增加了50%，在转基因植物相比，对照植物转化的二元载体没有插入（PZP 111）。初步的GPX过表达植物的二维电泳蛋白质分析表明，显着减少的蛋白质的积累被确定为rubisco小亚基I和果糖-1，6-二磷酸醛缩酶，两种蛋白质参与光合作用。这些观察结果，以及在标准培养条件下，GPX过表达植物与对照植物在表型上没有区别的事实，促使我们用已知会影响光合作用活性的冷冻处理来挑战植物。我们发现，在低温处理与弱光水平，光合作用不受影响GPX过表达的植物，而在对照植物，所揭示的叶绿素荧光参数和果糖-1，6-二磷酸酶活性。这些结果表明，在番茄植物中的硒非依赖性GPX的过表达修改特定的基因表达，并导致光合调节过程的修改。(c)2005 Elsevier B.V保留所有权利。
To investigate the function of glutathione peroxidase (GPX) in plants, we produced transgenic tomato plants overexpressing an eukaryotic selenium-independent GPX (GPX5). We show here that total GPX activity was increased by 50% in transgenic plants, when compared to control plants transformed with the binary vector without the insert (PZP111). A preliminary two-dimensional electrophoretic protein analysis of the GPX overexpressing plants showed notably a decrease in the accumulation of proteins identified as rubisco small subunit I and fructose-1,6-bisphosphate aldolase, two proteins involved in photosynthesis. These observations, together with the fact that in standard culture conditions, GPX-overexpressing plants were not phenotypically distinct from control plants prompted us to challenge the plants with a chilling treatment that is known to affect photosynthesis activity. We found that upon chilling treatment with low light level, photosynthesis was not affected in GPX-overexpressing plants while it was in control plants, as revealed by chlorophyll fluorescence parameters and fructose-1,6-biphosphatase activity. These results suggest that overexpression of a selenium-independent GPX in tomato plants modifies specifically gene expression and leads to modifications of photosynthetic regulation processes. (c) 2005 Elsevier B.V All rights reserved.