Modeling PNS and CNS Myelination Using Microfluidic Chambers.
Modeling PNS and CNS Myelination Using Microfluidic Chambers.
复制标题
使用微流体室模拟 PNS 和 CNS 髓鞘形成。
作者：
Adrien Vaquié;Alizée Sauvain;C. Jacob
体外髓鞘形成模型可以对发育髓鞘形成和短期髓鞘维持进行机制研究，但由于细胞密度高以及神经元和髓鞘细胞之间缺乏分离，使用现有模型进行的分析通常受到限制。此外，损伤后有髓鞘系统的再生研究需要神经元细胞体、轴突和髓鞘细胞的区室化。在这里，我们描述了一种使用微流体的分区方法，该方法允许在单细胞水平上进行活细胞成像，以跟踪神经元和髓鞘细胞的短期和长期动态相互作用以及大规模分析，例如分别对纯或高度富集的神经元或髓鞘细胞进行RNA测序。
Modeling myelination in vitro allows mechanistic study of developmental myelination and short-term myelin maintenance, but analyses possible to carry out using currently available models are usually limited because of high cell density and the lack of separation between neurons and myelinating cells. Furthermore, regeneration studies of myelinated systems after lesion require compartmentalization of neuronal cell bodies, axons, and myelinating cells. Here we describe a compartmentalized method using microfluidics that allows live-cell imaging at the single-cell level to follow short- and long-term dynamic interactions of neurons and myelinating cells and large-scale analyses, e.g., RNA sequencing on pure or highly enriched neurons or myelinating cells, separately.