ELT-3:: A Caenorhabditis elegans GATA factor expressed in the embryonic epidermis during morphogenesis
ELT-3:: A Caenorhabditis elegans GATA factor expressed in the embryonic epidermis during morphogenesis
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DOI：
10.1006/dbio.1999.9202
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发表时间：
1999-04-15
影响因子：
2.7
通讯作者：
McGhee, JD
中科院分区：
文献类型：
--
作者：
Gilleard, JS;Shafi, Y;McGhee, JD
我们已经鉴定出编码秀丽隐杆线虫 GATA 转录因子家族新成员 elt-3 的基因。预测的ELT-3多肽含有单个GATA型锌指(C-X-2-C-X-17-C-X-2-C)以及保守的相邻碱性区域，elt-3 mRNA存在于秀丽隐杆线虫发育的所有阶段，但在胚胎中最丰富。报告基因分析和抗体染色表明，elt-3首先在背侧和腹侧皮下细胞以及头部和尾部的皮下细胞中表达，紧接在产生这些细胞的最终胚胎细胞分裂之后。在外侧皮下（缝）细胞中未观察到表达。 elt-3的表达在表皮中维持在恒定水平，直至发育的2 1/2倍阶段，此后报告基因表达下降至低水平，并且不再能被特异性抗体检测到内源蛋白。紧邻肠道前后的细胞中 elt-3 表达的第二阶段始于椒盐卷饼阶段胚胎，elt-1 和 lin-26 是已知在皮下细胞命运的规范和维持中发挥重要作用的两个基因。我们发现大多数（但不是全部）表达 elt-3 的细胞的形成需要 elt-1。相反，lin-26 函数对于 elt-3 表达来说似乎不是必需的。最后，我们鉴定了线虫 Caenorhabditis briggsae 中 elt-3 的候选同源物。 elt-3 基因组和转录本结构的许多特征在两个物种之间是保守的，这表明 elt-3 可能在发育过程中发挥重要的进化功能。 (C) 1999 年学术出版社。
We have identified a gene encoding a new member of the Caenorhabditis elegans GATA transcription factor family, elt-3. The predicted ELT-3 polypeptide contains a single GATA-type zinc finger (C-X-2-C-X-17-C-X-2-C) along with a conserved adjacent basic region, elt-3 mRNA is present in all stages of C. elegans development but is most abundant in embryos. Reporter gene analysis and antibody staining show that elt-3 is first expressed in the dorsal and ventral hypodermal cells, and in hypodermal cells of the head and tail, immediately after the final embryonic cell division that gives rise to these cells. No expression is seen in the lateral hypodermal (seam) cells. elt-3 expression is maintained at a constant level in the epidermis until the 2 1/2-fold stage of development, after which reporter gene expression declines to a low level and endogenous protein can no longer be detected by specific antibody. A second phase of elt-3 expression in cells immediately anterior and posterior to the gut begins in pretzel-stage embryos, elt-1 and lin-26 are two genes known to be important in specification and maintenance of hypodermal cell fates. We have found that elt-1 is required for the formation of most, but not all, elt-3-expressing cells. In contrast, lin-26 function does not appear necessary for elt-3 expression. Finally, we have characterised the candidate homologue of elt-3 in the nematode Caenorhabditis briggsae. Many features of the elt-3 genomic and transcript structure are conserved between the two species, suggesting that elt-3 is likely to perform an evolutionarily significant function during development. (C) 1999 Academic Press.