Characterization of Saccharomyces cerevisiae deficient in expression of phospholipase D.
Characterization of Saccharomyces cerevisiae deficient in expression of phospholipase D.
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缺乏磷脂酶 D 表达的酿酒酵母的表征。
DOI：
10.1042/bj3140015
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Meier,KE
中科院分区：
文献类型：
--
作者：
Ella,KM;Dolan,JW;Qi,C;Meier,KE
在酿酒酵母中发现了一个编码磷脂酶D （PLD）的基因。PLD1的195 kDa产物与植物PLD的整体序列同源性为24%。酵母PLD活性的表达通过一步基因破坏被消除。缺乏PLD活性的酵母单倍体在不可发酵碳源上生长不足。缺乏PLD1表达的二倍体不能产孢。
A gene encoding phospholipase D (PLD) in Saccharomyces cerevisiae was identified. The 195 kDa product of PLD1 has 24% overall sequence identity with a plant PLD. Expression of yeast PLD activity was eliminated by one-step gene disruption. Yeast haploids lacking PLD activity were deficient in growth on non-fermentable carbon sources. Diploids lacking expression of PLD1 were unable to sporulate.