Preparation of trisaccharides from alginate by a novel alginate lyase Alg7A from marine bacterium Vibrio sp. W13
Preparation of trisaccharides from alginate by a novel alginate lyase Alg7A from marine bacterium Vibrio sp. W13
复制标题
利用来自海洋细菌弧菌的新型藻酸盐裂解酶 Alg7A 从藻酸盐制备三糖。
DOI：
10.1016/j.ijbiomac.2019.08.020
复制
发表时间：
2019
影响因子：
8.2
通讯作者：
Yin Heng
中科院分区：
文献类型：
--
作者：
Zhu Benwei;Li Kuikui;Wang Wenxia;Ning Limin;Tan Haidong;Zhao Xiaoming;Yin Heng
酶法消化海藻酸钠生产特定的低聚糖引起了人们的广泛关注。然而，有效地生产特定低聚糖的商业酶仍然不可用。在本研究中，从海洋细菌弧菌中克隆了一个新的编码海藻酸裂解酶的基因（命名为alg7a）。W13表达inE。杆菌。重组Alg7A对海藻酸盐、聚α-l-谷露醛酸酯（polyG）、聚β-d-甘露醛酸酯（polyM）和聚mg均表现出较高的活性，且对聚mg的活性更强。此外，该酶含有多糖裂解酶（PL） 7家族的一个高度保守结构域（R*E*R， Q*H和Y*KAG*Y*Q），这表明它属于PL7。薄层色谱和ESI-MS分析表明，Alg7A主要从海藻酸盐中释放三糖。这些结果表明，Alg7A具有利用海藻酸盐生产低聚糖的巨大潜力。
Enzymatic digestion of sodium alginate to produce specific oligosaccharides has attracted great attention. However, commercial enzymes that efficiently produce specific oligosaccharides are still unavailable. In the present study, a novel gene encoding an alginate lyase (designatedalg7A) was cloned from the marine bacteriumVibriosp. W13 and expressed inE. coli. The recombinant Alg7A shows high activities toward alginate, poly-α-l-guluronate (polyG), poly-β-d-mannuronate (polyM) and polyMG, and more preferred to polyMG. Moreover, the enzyme contains a highly conserved domain of the Polysaccharide Lyase (PL) 7 family (R*E*R, Q*H and Y*KAG*Y*Q), which indicates that it belongs to PL7. Furthermore, the thin layer chromatography and ESI-MS analysis showed that Alg7A mainly releases trisaccharides from alginate. These results demonstrated that Alg7A has a great potential to be used to produce oligosaccharides from alginate.