Translational control of the embryonic cell cycle
Translational control of the embryonic cell cycle
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DOI：
10.1016/s0092-8674(02)00733-x
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发表时间：
2002-05-17
期刊：
影响因子：
64.5
通讯作者：
Richter, JD
中科院分区：
文献类型：
--
作者：
Groisman, I;Jung, MY;Richter, JD
细胞周期蛋白B的合成和破坏驱动真核细胞的有丝分裂。细胞周期进程也在细胞周期蛋白B翻译水平上受到调节。在爪蟾胚胎的循环提取物中，进入M期需要多腺苷化诱导的细胞周期蛋白B1 mRNA的翻译。多聚腺苷化是由极光激酶介导的CPEB磷酸化介导的，极光激酶的活性随细胞周期振荡。从M期退出似乎需要细胞周期蛋白B1 mRNA被Maskin（一种CPEB和eIF4E结合因子，其表达受细胞周期调控）死烯化和随后的翻译沉默。这些观察结果表明，受调节的细胞周期蛋白B1 mRNA翻译对胚胎细胞周期至关重要。哺乳动物细胞也表现出细胞周期依赖的胞质聚腺苷化，这表明由聚腺苷化控制的翻译可能是动物细胞有丝分裂的一个普遍特征。
The synthesis and destruction of cyclin B drives mitosis in eukaryotic cells. Cell cycle progression is also regulated at the level of cyclin B translation. In cycling extracts from Xenopus embryos, progression into M phase requires the polyadenylation-induced translation of cyclin B1 mRNA. Polyadenylation is mediated by the phosphorylation of CPEB by Aurora, a kinase whose activity oscillates with the cell cycle. Exit from M phase seems to require deadenylation and subsequent translational silencing of cyclin B1 mRNA by Maskin, a CPEB and eIF4E binding factor, whose expression is cell cycle regulated. These observations suggest that regulated cyclin B1 mRNA translation is essential for the embryonic cell cycle. Mammalian cells also display a cell cycle-dependent cytoplasmic polyadenylation, suggesting that translational control by polyadenylation might be a general feature of mitosis in animal cells.