The global regulatory effect of Edwardsiella tarda Fur on iron acquisition, stress resistance, and host infection: A proteomics-based interpretation
The global regulatory effect of Edwardsiella tarda Fur on iron acquisition, stress resistance, and host infection: A proteomics-based interpretation
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迟缓爱德华氏菌毛皮对铁获取、应激抵抗和宿主感染的全球调节作用：基于蛋白质组学的解释
DOI：
10.1016/j.jprot.2016.04.005
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发表时间：
2016-05-17
影响因子：
3.3
通讯作者：
Sun, Li
中科院分区：
文献类型：
--
作者：
Hu, Yong-hua;Sun, Li
铁吸收调节因子（Fur）是革兰氏阴性菌重要的转录调节因子。迟缓爱德华氏菌是一种严重的鱼类细菌病原体，具有广泛的宿主范围，包括人类。在本研究中，我们研究了Fur在大肠杆菌中的调节功能。通过蛋白质组学的方法。与野生型TX01相比，毛皮突变体TX01 Delta毛皮表现出（i）生长迟缓，（ii）铁载体产生增强，（iii）耐酸性增加，这与其他细菌物种中的观察结果相反，（iv）抗氧化应激和宿主血清的存活减少，（v）抑制宿主免疫应答的能力受损，（vi）组织感染性和总体毒力减弱。通过导入外源性fur基因，挽救了TX01 Delta fur的缺陷。基于iTRAQ的TX01 Delta毛皮和TX01的比较蛋白质组学分析鉴定了89种差异表达的蛋白质，这些蛋白质涵盖了广泛的功能类别，包括受毛皮突变影响的蛋白质。此外，在革兰氏阴性菌中首次鉴定出16个受Fur调控的蛋白。这些结果提供了第一个基于蛋白质的解释的全球影响毛皮的生理和感染性的E。tarda.意义：本研究表明，在E. Tarda，Fur控制细菌生命的多个方面，包括生长，代谢，铁的获取，应激反应和宿主感染。与这些观察结果一致，蛋白质组学分析鉴定了大量受Fur影响的蛋白质表达，这些蛋白质参与细菌生理学和感染性。因此，这些结果首次将Fur的多效性效应与全局蛋白表达联系起来，并为Fur在病原菌中的功能和调控机制提供了新的见解。(C)2016由Elsevier B.V.出版
Ferric uptake regulator (Fur) is an important transcriptional regulator of Gram-negative bacteria. Edwardsiella tarda is a severe fish bacterial pathogen with a broad host range that includes humans. In this study, we examined the regulatory function of Fur in E. tarda via a proteomic approach. Compared to the wild type TX01, the fur mutant TX01 Delta fur exhibited (i) retarded growth, (ii) enhanced siderophore production, (iii) increased acid tolerance, which is in contrast to observations in other bacterial species, (iv) decreased survival against oxidative stress and host serum, (v) impaired ability to inhibit host immune response, (vi) attenuated tissue infectivity and overall virulence. The deficiency of TX01 Delta fur was rescued by introduction of an exogenous fur gene. iTRAQ-based comparative proteomic analysis of TX01 Delta fur and TX01 identified 89 differentially expressed proteins that cover a wide range of functional categories including those affected by fur mutation. In addition, 16 proteins were identified for the first time to be regulated by Fur in Gram-negative bacteria. These results provide the first protein based interpretation of the global impact of Fur on the physiology and infectivity of E. tarda.Significance: This study demonstrates that in E. tarda, Fur controls multiple aspects of bacterial life, including growth, metabolism, iron acquisition, stress response, and host infection. In line with these observations, proteomics analysis identified a large amount of proteins affected in expression by Fur, which are involved in bacterial physiology and infectivity. Hence, these results link for the first time the pleiotropic effect of Fur with global protein expression and shed new light on the function and regulatory mechanism of Fur in pathogenic bacteria. (C) 2016 Published by Elsevier B.V.