Phylogenetic and virulence analysis of tick-borne encephalitis viruses from Japan and far-eastern Russia
Phylogenetic and virulence analysis of tick-borne encephalitis viruses from Japan and far-eastern Russia
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DOI：
10.1099/0022-1317-80-12-3127
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发表时间：
1999-12-01
影响因子：
3.8
通讯作者：
Takashima, I
中科院分区：
文献类型：
--
作者：
Hayasaka, D;Suzuki, Y;Takashima, I
我们以前曾报道过蜱传脑炎（TBE）在日本北海道的一个特定地区流行。在北海道南部的大岛，1995年和1996年从哨兵犬、蜱和啮齿动物中分离出TEE病毒。为了确定这些TEE病毒何时在北海道出现，估计了在大岛和俄罗斯远东分离的TEE病毒株的分歧时间。1998年在哈巴罗夫斯克分离到TEE病毒，并比较了来自大岛和哈巴罗夫斯克的分离株的病毒包膜蛋白基因的核苷酸序列。从这些病毒株的同义替换率来看，这些TEE病毒株的谱系分歧时间在遗传学上预测为大约260-430年前。此外，在小鼠模型中比较了来自大岛和哈巴罗夫斯克的TEE病毒分离株的毒力。结果表明，分离株对小鼠的毒力非常相似。本报告提供的证据表明，北海道的TEE病毒大岛株是几百年前从俄罗斯远东地区出现的，这解释了为什么这些毒株具有与俄罗斯分离的TEE病毒相似的毒力。
We have previously reported that tick-borne encephalitis (TBE) is endemic in a specific area of Hokkaido, Japan. In Oshima, the southern part of Hokkaido, TEE virus was isolated from sentinel dogs, ticks and rodents in 1995 and 1996. To identify when these TEE viruses emerged in Hokkaido, the times of divergence of TEE virus strains isolated in Oshima and far-eastern Russia were estimated. TEE virus was isolated in Khabarovsk in 1998 and the nucleotide sequences of viral envelope protein genes of isolates from Oshima and Khabarovsk were compared. From the synonymous substitution rate of these virus strains, the lineage divergence time of these TEE virus strains was predicted phylogenetically to be about 260-430 years ago. Furthermore, the virulence of TEE virus isolates from Oshima and Khabarovsk were compared in a mouse model. The results showed that the isolates possessed very similar virulence in mice. This report provides evidence that the Oshima strains of TEE virus in Hokkaido emerged from far-eastern Russia a few hundred years ago and this explains why these strains possess virulence similar to the TEE viruses isolated in Russia.