Smooth muscle of the dorsal aorta shares a common clonal origin with skeletal muscle of the myotome
Smooth muscle of the dorsal aorta shares a common clonal origin with skeletal muscle of the myotome
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DOI：
10.1242/dev.02226
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发表时间：
2006-02-01
期刊：
影响因子：
4.6
通讯作者：
Buckingham, ME
中科院分区：
文献类型：
--
作者：
Esner, M;Meilhac, SM;Buckingham, ME
我们发现，背主动脉（一种早期血管）和肌节（体节内形成的第一个骨骼肌）的细胞源自小鼠胚胎中的共同祖细胞。这一结论基于使用针对 α-心脏肌动蛋白基因的 nlaacZ 报告基因进行的回顾性克隆分析。罕见的基因内重组事件产生功能性 nlacZ 序列，从而产生 β-半乳糖苷酶阳性细胞的克隆。背主动脉的内皮周细胞和血管平滑肌细胞是标记的主要细胞类型，表明这些细胞与骨骼肌的近轴中胚层衍生细胞有克隆相关。在一些克隆中还可以看到罕见的内皮细胞。在由最近的重组事件产生的较年轻的克隆中，肌节标记主要位于轴下体节，邻近主动脉中标记的平滑肌细胞。对 Pax3(GFP/+) 胚胎的分析表明，这些细胞是 Pax3 阴性但 GFP 阳性，在主动脉和体节之间的中间区域有荧光细胞。这与表达 Pax3 的平滑肌前体细胞的直接迁移一致。这些结果根据轴旁中胚层对主动脉的贡献以及从中衍生的中成血管细胞干细胞进行了讨论。
We show that cells of the dorsal aorta, an early blood vessel, and of the myotome, the first skeletal muscle to form within the somite, derive from a common progenitor in the mouse embryo. This conclusion is based on a retrospective clonal analysis, using a nlaacZ reporter targeted to the alpha-cardiac actin gene. A rare intragenic recombination event results in a functional nlacZ sequence, giving rise to clones of beta-galactosidase-positive cells. Periendothelial and vascular smooth muscle cells of the dorsal aorta are the main cell types labelled, demonstrating that these are clonally related to the paraxial mesoderm-derived cells of skeletal muscle. Rare endothelial cells are also seen in some clones. in younger clones, arising from a recent recombination event, myotomal labelling is predominantly in the hypaxial somite, adjacent to labelled smooth muscle cells in the aorta. Analysis of Pax3(GFP/+) embryos shows that these cells are Pax3 negative but GFP positive, with fluorescent cells in the intervening region between the aorta and the somite. This is consistent with the direct migration of smooth muscle precursor cells that had expressed Pax3. These results are discussed in terms of the paraxial mesoderm contribution to the aorta and of the mesoangioblast stem cells that derive from it.