Interactive cloning with the SH3 domain of N-src identifies a new brain specific ion channel protein, with homology to Eag and cyclic nucleotide-gated channels
Interactive cloning with the SH3 domain of N-src identifies a new brain specific ion channel protein, with homology to Eag and cyclic nucleotide-gated channels
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DOI：
10.1073/pnas.94.26.14815
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发表时间：
1997-12-23
影响因子：
11.1
通讯作者：
Kandel, ER
中科院分区：
文献类型：
--
作者：
Santoro, B;Grant, SGN;Kandel, ER
我们通过使用酵母双杂交系统和 Src (N-src) 神经形式的 SH3 结构域作为诱饵，分离出了一种新的 cDNA，它似乎代表了一类新的离子通道。编码的多肽 BCNG-1 与环核苷酸门控通道和电压门控通道 Eag 和 H-erg 关系较远，BCNG-1 仅在大脑中表达，为约 132 kDa 的糖基化蛋白。免疫组织化学分析表明 BCNG-1 优先在新皮质、海马和小脑的特定神经元亚群中表达，特别是锥体神经元和篮细胞。在单个神经元内，BCNG-1 蛋白根据细胞类型定位于树突或轴突末端。 Southern印迹分析显示小鼠基因组中存在其他几个BCNG相关序列，表明离子通道编码基因的整个亚家族的出现。这些发现表明存在一种新型离子通道，它可能能够调节大脑中的膜兴奋性，并可能对环核苷酸的调节做出反应。
We have isolated a novel cDNA, that appears to represent a new class of ion channels, by using the yeast two-hybrid system and the SH3 domain of the neural form of Src (N-src) as a bait. The encoded polypeptide, BCNG-1, is distantly related to cyclic nucleotide-gated channels and the voltage-gated channels, Eag and H-erg, BCNG-1 is expressed exclusively in the brain, as a glycosylated protein of approximate to 132 kDa. Immunohistochemical analysis indicates that BCNG-1 is preferentially expressed in specific subsets of neurons in the neocortex, hippocampus, and cerebellum, in particular pyramidal neurons and basket cells. Within individual neurons, the BCNG-1 protein is localized to either the dendrites or the axon terminals depending on the cell type. Southern blot analysis shows that several other BCNG-related sequences are present in the mouse genome, indicating the emergence of an entire subfamily of ion channel coding genes. These findings suggest the existence of a new type of ion channel, which is potentially able to modulate membrane excitability in the brain and could respond to regulation by cyclic nucleotides.