Inhibition of human parainfluenza virus-3 replication by interferon and human MxA.
Inhibition of human parainfluenza virus-3 replication by interferon and human MxA.
复制标题
干扰素和人 MxA 抑制人副流感病毒 3 复制。
DOI：
10.1006/viro.1996.0321
复制
发表时间：
1996
期刊：
影响因子：
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通讯作者：
Banerjee,AK
中科院分区：
文献类型：
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作者：
Zhao,H;De,BP;Das,T;Banerjee,AK
我们研究了干扰素在培养的人A549细胞中对人副流感病毒3型(HPIV-3)复制的抑制作用。干扰素-α显著抑制病毒产量，病毒积累减少90%以上。进一步的研究表明，干扰素的抑制作用是在HPIV3复制的初级转录水平。由于干扰素诱导蛋白MxA已被证明在几种RNA病毒中抑制病毒复制，我们使用稳定表达MxA的人胶质母细胞瘤细胞系检测了MxA在HPIV-3复制中的作用。在这些细胞中，HPIV-3复制减少了100多倍，这取决于使用的病毒剂量，同时伴随着对病毒RNA合成的抑制约80%。然而，在该产生MXA的细胞系中，病毒的初级转录没有受到影响。相反，在亲代细胞系中，干扰素介导的抑制发生在HPIV-3复制的初级转录步骤。这些数据表明，除了MxA，在所用的两种细胞系中，干扰素还参与了其他诱导蛋白的抗HPIV-3作用。
We have investigated the IFN-mediated inhibition of human parainfluenza virus-3 (HPIV-3) replication in cultured human A549 cells. IFN-α inhibited the virus yield significantly with concomitant reduction of viral RNA accumulation by more than 90%. Further studies indicated that the inhibitory action of IFN was at the level of primary transcription of HPIV3 replication. Since the IFN-inducible protein, MxA, has been shown to inhibit virus replication in several RNA viruses, we examined the role of MxA in HPIV-3 replication using a stably transfected human glioblastoma cell line expressing MxA. In these cells HPIV-3 replication was decreased by more than 100-fold depending on the virus dosage used with concomitant inhibition of viral RNA synthesis by about 80%. However, the viral primary transcription was not affected in this MxA-producing cell line. In contrast, in the parental cell line IFN-mediated inhibition occurred at the primary transcription step of HPIV-3 replication. These data suggest that, in addition to MxA, other IFN-inducible proteins are involved in the anti-HPIV-3 effect of IFN in both the cell lines used.