Proteomic analysis of slow- and fast-twitch skeletal muscles
Proteomic analysis of slow- and fast-twitch skeletal muscles
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DOI：
10.1002/pmic.200401181
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发表时间：
2005-07-01
期刊：
影响因子：
3.4
通讯作者：
Nagai, K
中科院分区：
文献类型：
--
作者：
Okumura, N;Hashida-Okumura, A;Nagai, K
骨骼肌由慢肌纤维和快肌纤维组成，它们分别在有氧和无氧ATP生产中具有高潜力。为了研究其功能差异的分子基础，我们使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和pH 4-7和6-11等电聚焦凝胶的二维凝胶电泳检测了骨骼肌的蛋白质谱。大鼠比目鱼肌和趾长伸肌（趾长伸肌）肌肉，主要是慢，快收缩纤维，分别进行比较，结果表明，趾长伸肌肌肉有较高水平的糖原磷酸化酶，大多数糖酵解酶，甘油3-磷酸脱氢酶，肌酸激酶：而比目鱼肌有较高水平的肌红蛋白，TCA循环酶，电子转移黄素蛋白，和碳酸酐酶III。这两块肌肉还表达了不同的收缩蛋白亚型，包括肌球蛋白重链和轻链。将这些蛋白质模式与红色和白色腓肠肌以及红色和白色股四头肌的蛋白质模式进行进一步比较。发现代谢酶显示出依赖于肌纤维类型的协同调节。另一方面，收缩蛋白的表达似乎与肌纤维的代谢特征无关。这些结果表明，代谢酶和收缩蛋白在骨骼肌中表现出不同的表达模式。
Skeletal muscles are composed of slow- and fast-twitch muscle fibers, which have high potential in aerobic and anaerobic ATP production, respectively. To investigate the molecular basis of the difference in their functions, we examined protein profiles of skeletal muscles using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and two-dimensional gel electrophoresis with pH 4-7 and 6-11 isoelectric focusing gels. A comparison between rat soleus and extensol digitorum longus (EDL) muscles that are predominantly slow- and fast-twitch fibers, respectively, showed that the EDL muscle had higher levels of glycogen phosphorylase, most glycolytic enzymes, glycerol 3-phosphate dehydrogenase, and creatine kinase: while the soleus muscle had higher levels of myoglobin, TCA cycle enzymes, electron transfer flavoprotein, and carbonic anhydrase III. The two muscles also expressed different isoforms of contractile proteins including myosin heavy and light chains. These protein patterns were further compared with those of red and white gastrochnemius as well as red and white quadriceps muscles. it was found that metabolic enzymes showed a concerted regulation dependent on muscle fiber types. On the other hand, expression of contractile proteins seemed to be independent of the metabolic characteristics of muscle fibers. These results suggest that metabolic enzymes and contractile proteins show different expression patterns in skeletal muscles.