Determination of acyl homoserine lactone and tetramic acid concentrations in biological samples.
Determination of acyl homoserine lactone and tetramic acid concentrations in biological samples.
复制标题
生物样品中酰基高丝氨酸内酯和特特拉姆酸浓度的测定。
DOI：
10.1007/978-1-60761-971-0_8
复制
发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Janda,KimD
中科院分区：
文献类型：
--
作者：
Lowery,ColinA;Kaufmann,GunnarF;Janda,KimD
在环境群落中，由于营养物质往往有限，人们不断为生存而斗争。作为回应，细菌已经开发出复杂的方法来对付竞争对手。其中一种机制是通过在称为群体感应的过程中交换小的化学信号来协调行为和功能。这一过程在铜绿假单胞菌的致病性中尤其突出，铜绿假单胞菌是一种机会性人类病原体，形成被称为生物膜的固着群落。这些生物膜在对虾的生活方式中起着重要作用。铜绿假单胞菌，无论是在其自然环境中还是在人类宿主中建立和维持感染期间;因此，它们经常对人类健康产生严重影响。因此，用于检测这些QS信号的方法可以提供对P的致病性和存活的见解。铜绿。在本章中，我们提出了一种方法用于提取和定量的P。aerosaQS信号肽N-3-氧代-十二烷酰基-高丝氨酸内酯及其重排的特特拉姆酸产物C12-TA，其本身具有作为P.铜绿。
Within environmental communities, there is a constant struggle for survival, as nutrients are often limited. In response, bacteria have developed elaborate methods to deal with competitors. One such mechanism is the coordination of behaviors and function via the exchange of small chemical signals in a process known as quorum sensing. This process is especially prominent in the pathogenicity ofPseudomonas aeruginosa, an opportunistic human pathogen that forms sessile communities known as biofilms. These biofilms play an important role in the lifestyle ofP. aeruginosa, either in their natural environment or during establishment and maintenance of infection in human hosts; thus, they often have grievous effects on human health. As such, a method for the detection of these QS signals may provide insights into the pathogenicity and survival ofP. aeruginosa. In this chapter, we present a method for the extraction and quantitation of theP. aeruginosaQS signalN-3-oxo-dodecanoyl-homoserine lactone, and its rearranged tetramic acid product, C12-TA, which itself has implications as a survival tactic used byP. aeruginosa.