HIGH PLASMALOGEN AND ARACHIDONIC-ACID CONTENT OF CANINE MYOCARDIAL SARCOLEMMA - A FAST ATOM BOMBARDMENT MASS SPECTROSCOPIC AND GAS-CHROMATOGRAPHY MASS SPECTROSCOPIC CHARACTERIZATION
HIGH PLASMALOGEN AND ARACHIDONIC-ACID CONTENT OF CANINE MYOCARDIAL SARCOLEMMA - A FAST ATOM BOMBARDMENT MASS SPECTROSCOPIC AND GAS-CHROMATOGRAPHY MASS SPECTROSCOPIC CHARACTERIZATION
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DOI：
10.1021/bi00296a026
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发表时间：
1984-01-01
期刊：
影响因子：
2.9
通讯作者：
GROSS, RW
中科院分区：
文献类型：
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作者：
GROSS, RW
纯化犬心肌肌膜，采用气相色谱-质谱法、快原子轰击质谱法和常规技术测定其磷脂成分。犬心肌肌膜含有2.7μmol脂质Pi/mg蛋白质，其主要由胆碱甘油磷脂(47％)、乙醇胺甘油磷脂(28％)和鞘磷脂(11％)组成。肌膜磷脂含有40%缩醛磷脂，其定量由胆碱（胆碱甘油磷脂的57%）和乙醇胺（乙醇胺甘油磷脂的64%）缩醛磷脂所占。胆碱缩醛磷脂主要含有棕榈醛的乙烯基醚，而乙醇胺缩醛磷脂主要由硬脂醛和油醛的乙烯基醚组成。大多数肌膜乙醇胺甘油磷脂 (75%) 含有与 sn-2 碳酯化的花生四烯酸。鞘磷脂主要由长链饱和脂肪酸（硬脂酸和花生酸）以及大量（8%）的奇数链长饱和脂肪酸组成。讨论了这些不寻常的磷脂成分可能的功能作用。
Canine myocardial sarcolemma was purified, and its phospholipid constituents were determined by gas chromatography-mass spectrometry, fast atom bombardment mass spectrometry and conventional techniques. Canine myocardial sarcolemma contained 2.7 .mu.mol of lipid Pi/mg of protein which was comprised predominantly of choline glycerophospholipids (47%), ethanolamine glycerophospholipids (28%) and sphingomyelin (11%). Sarcolemmal phospholipids contained 40% plasmalogen which was quantitatively accounted for by choline (57% of choline glycerophospholipid) and ethanolamine (64% of ethanolamine glycerophospholipid) plasmalogens. Choline plasmalogens contained predominantly the vinyl ether of palmitic aldehyde though ethanolamine plasmalogens were composed predominantly of the vinyl ethers of stearic and oleic aldehydes. The majority of sarcolemmal ethanolamine glycerophospholipids (75%) contained arachidonic acid esterified to the sn-2 carbon. Sphingomyelin was composed predominantly of long-chain saturated fatty acids (stearic and arachidic) as well as substantial amounts (8%) of odd chain length saturated fatty acids. The possible functional role of these unusual phospholipid constituents is discussed.