Genetic linkage map of Lolium multiflorum Lam. constructed from a BC1 population derived from an interspecific hybridization, L. multiflorum x Lolium temulentum L. x L. temulentum
Genetic linkage map of Lolium multiflorum Lam. constructed from a BC1 population derived from an interspecific hybridization, L. multiflorum x Lolium temulentum L. x L. temulentum
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DOI：
10.1111/grs.12055
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发表时间：
2014-09-01
影响因子：
1.3
通讯作者：
Cai, Hongwei
中科院分区：
文献类型：
--
作者：
Guan, Xuanli;Hirata, Mariko;Cai, Hongwei
利用从禾谷类植物表达序列标签序列中开发的SSR标记和保守内含子扫描引物（CISP）标记成功构建了多花黑麦草（Lolium multiflorum Lam.）遗传连锁图谱。使用L.多花黑麦草x L. temulentum BC 1群体由88个个体组成。该连锁图包括108个SSR标记和84个CISP标记，覆盖488.8cM，分布在7个连锁群中，长度范围为42.2 ~ 98.9cM。标记的平均密度为2.5cM。黑麦草属与水稻的同线关系基于CISP标记的位置，在很大程度上同意以前报道的关系。本研究所构建的遗传连锁图将有助于构建一个更密集的连锁图，以及对重要农艺性状的数量性状位点的分析。
Simple sequence repeat (SSR) markers and conserved-intron scanning primer (CISP) markers developed from cereal expressed sequence tag sequences were successfully used to construct a linkage map for Lolium multiflorum Lam. using a L. multiflorum x Lolium temulentum L. x L. temulentum BC1 population consisting of 88 individuals. The genetic linkage map included 108 SSR and 84 CISP markers that covered 488.8 centimorgan (cM) in seven linkage groups, ranging in length from 42.2 to 98.9 cM. The average density of markers was 2.5 cM. Syntenic relationships between Lolium and rice (Oryza sativa L.) based on the positions of the CISP markers largely agree with the previously reported relationships. The map constructed in the present study will be useful in developing a denser linkage map as well as for analysis of quantitative trait loci for important agronomic traits in Lolium species.