GENETIC MAPPING OF PROPHAGE MU
GENETIC MAPPING OF PROPHAGE MU
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DOI：
10.1016/0042-6822(73)90120-7
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发表时间：
1973-01-01
期刊：
影响因子：
3.7
通讯作者：
METLAY, M
中科院分区：
文献类型：
--
作者：
BUKHARI, AI;METLAY, M
几个独立分离的Mu原噬菌体的基因顺序已被确定的缺失作图。产生缺失的原噬菌体位于大肠杆菌的lacI基因、lacZ基因和troperon。针对一组16个Mu琥珀突变体测试Mu缺失的标志物拯救。通过构建隐蔽Mu原噬菌体和Mu琥珀突变体的溶原性菌株，然后检查所得的杂合基因（Mu的部分二倍体）自发产生野生型Mu颗粒，进行标记拯救试验。所有穆原噬菌体的基因顺序是相同的，虽然原噬菌体被发现在两个方向相对于主机标记。因此可以推断，随机插入到大肠杆菌染色体上的温和噬菌体Mu。大肠杆菌，使用其自身DNA上的特定位点进行整合。
The gene order of several independently isolated Mu prophages has been determined by deletion mapping. The prophages in which deletions were produced were located in thelacIgene, thelacZgene, and thetrpoperon ofEscherichia coli. The Mu deletions were tested for marker rescue against a set of sixteen Mu amber mutants. Marker rescue tests were done by constructing strains lysogenic for both cryptic Mu prophages and Mu amber mutants and then examining the resulting heterogenotes, partially diploid for Mu, for spontaneous production of wild-type Mu particles. The gene order of all Mu prophages was identical, although prophages were found in either of the two orientations with respect to the host markers. It can be inferred therefore that the temperate bacteriophage Mu, which inserts randomly into the chromosome ofE. coli, uses a specific site on its own DNA for integration.