Evaluation of the polymorphism in the Toll-like receptor 4 (TLR4) genes of tympanosclerosis patients
Evaluation of the polymorphism in the Toll-like receptor 4 (TLR4) genes of tympanosclerosis patients
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DOI：
10.1016/j.anl.2009.03.001
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发表时间：
2010-02-01
影响因子：
1.7
通讯作者：
Yalcin, Sinasi
中科院分区：
文献类型：
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作者：
Alpay, Hayrettin Cengiz;Etem, Ebru Onalan;Yalcin, Sinasi
目的：虽然鼓膜穿孔和中耳感染被认为是引起鼓膜炎（TS）的原因，但慢性中耳炎（COM）中也会出现鼓膜炎，但有些慢性中耳炎（COM）持续存在，穿孔持续时间长，但并不出现鼓膜炎。在这项研究中，显示TS形成的遗传差异的分子原因的影响进行了评估：我们的目的是确定Asp299Gly多态性频率在TLR4基因的TS患者谁有COM，和患者谁没有。材料和方法：选择已行COM手术的患者，随机分为两组，每组50人，在手术过程中，他们的中间车厢是否有TS。选择100例人口学资料相似的健康体检者作为对照组。采用标准方法对患病组和对照组外周血标本进行DNA提取。采用限制性片段长度多态性（RFLP）方法检测TLR4基因Asp299Gly多态性。结果：在TS患者组中，有5例患者存在TLR4 Asp299Gly多态性（10%）。TLR4 Asp299Gly多态性在无TS组中仅1例（2%）阳性。TLR4 Asp299Gly多态性在对照组100例中有6例（6%）阳性。TS组与非TS组比较，阳性多态性差异有统计学意义（p < 0.05）。结论：TS可能不存在于中耳感染、穿孔多年的患者中，但TS基因多态性的发生率高于对照组，差异无统计学意义（p > 0.05）。动脉粥样硬化中TLR4基因的多态性导致炎症细胞因子振荡识别细菌LPS，也被指责。从我们对有限数量的患者和仅一个基因的研究结果中发现，显示遗传差异的分子原因也可以有效地形成TS，这是引人入胜的。需要更大范围的系列研究。(C)2009爱思唯尔爱尔兰有限公司保留所有权利。
Objective: Although eardrum perforations which endure etiopathogenesis for a long-time and middle ear infections are proposed for causing the tympanosclerosis (TS), tympanosclerosis emerges in some chronic otitis media (COM), some of them do not appear although a continuing COM and enduring perforation last. In this study, the effect of the molecular reasons which display genetic differences in TS formation is evaluated: our aim is to determine the Asp299Gly polymorphism frequencies in the TLR4 gene of patients with TS who have COM, and patients who do not.Materials and methods: Patients who have undergone COM surgery, were divided into two groups of 50 persons who were selected in accordance with the fact, whether they had TS in their middle car cavity or not during operation. 100 healthy persons who had similar demographic data, were evaluated as the control group. The DNA isolation was executed by using standard methods with peripheric blood specimen of the diseased group and control group. The Restriction Fragment Length Polymorphism method was used in determining the Asp299Gly allel in the TLR4 gene. Items of 249 bc for the wild tip (Asp) post-restriction enzyme segment wild tip (Asp) allel, and 23 bc and 196 bc post-restriction enzyme segment polymorphic allel (Gly) were obtained.Results: TLR4 Asp299Gly polymorphism (10%) was asserted in a total of five specimens in the diseased group with TS. TLR4 Asp299Gly polymorphism was found positive in only one (2%) of the 50 phenomenons in the group without TS. TLR4 Asp299Gly polymorphism was found positive in six (6%) of the 100 phenomenons in the control group. The positive polymorphism in phenomenons with TS was significant in accordance with statistics, when compared with the group without TS (p < 0.05). However, although the polymorphism rates were higher than the rates of the control group, it was not statistically significant (p > 0.05).Conclusion: TS may not appear in many patients who had undergone middle ear infection, and had perforation for many years. The polymorphism in arteriosclerosis in the TLR4 gene which caused the inflammatory cytokines oscillation recognize the bacterial LPS, was also accused. It is engrossing to find out from the results of our study on a restricted number of patients, and on only one gene, that molecular reasons which display genetic differences can also be effective in forming TS. Serial researches of greater dimensions are required. (C) 2009 Elsevier Ireland Ltd. All rights reserved.