Simultaneous stimulation of RuBP regeneration and electron transport increases productivity and water use efficiency under field conditions
Simultaneous stimulation of RuBP regeneration and electron transport increases productivity and water use efficiency under field conditions
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DOI：
10.1101/2020.01.27.920843
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发表时间：
2020-01
期刊：
影响因子：
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通讯作者：
Patricia E. López-Calcagno;Kenny L. Brown;A. Simkin;Stuart J. Fisk;T. Lawson;C. Raines
中科院分区：
文献类型：
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作者：
Patricia E. López-Calcagno;Kenny L. Brown;A. Simkin;Stuart J. Fisk;T. Lawson;C. Raines
以往的研究表明，无论是电子传递或RuBP再生的独立刺激可以增加光合碳同化和植物生物量的速率。在本文中，我们提出的证据表明，多基因的方法，同时操纵这两个过程提供了进一步的刺激光合作用。我们报告的介绍蓝藻双功能酶果糖-1，6-二磷酸酶/景天庚酮糖-1，7-二磷酸酶或过表达的植物酶景天庚酮糖-1，7-二磷酸酶，以及表达的红藻蛋白细胞色素C6，并表明，在温室和田间条件下，可以实现生物量积累的进一步增加。此外，我们提供的证据表明，同时刺激电子传递和RuBP再生可以导致提高内在水分利用效率在现场条件下。一句话总结同时刺激RuBP再生和电子传递导致温室和田间生长的烟草的生物量产量的提高。
Previous studies have demonstrated that independent stimulation of either electron transport or RuBP regeneration can increase the rate of photosynthetic carbon assimilation and plant biomass. In this paper, we present evidence that a multi-gene approach to simultaneously manipulate these two processes provides a further stimulation of photosynthesis. We report on the introduction of the cyanobacterial bifunctional enzyme fructose-1,6-bisphosphatase/sedoheptulose-1,7-bisphosphatase or overexpression of the plant enzyme sedoheptulose-1,7-bisphosphatase, together with expression of the red algal protein cytochrome c6, and show that a further increase in biomass accumulation under both glasshouse and field conditions can be achieved. Furthermore, we provide evidence that the simultaneous stimulation of electron transport and RuBP regeneration can lead to enhanced intrinsic water use efficiency under field conditions. One sentence summary Simultaneous stimulation of RuBP regeneration and electron transport results in improvements in biomass yield in glasshouse and field grown tobacco.